复方五仁醇胶囊抗氧化及抑制肝细胞凋亡的血清药理学研究

目的:研究复方五仁醇胶囊保肝作用的机理。方法:大鼠灌胃给予复方五仁醇胶囊制备含药血清,CCl4诱导原代培养大鼠肝细胞损伤模型,测定含药血清作用后细胞培养液中丙二醛(MDA)含量和超氧化物岐化酶(SOD)活性,Annexin Ⅴ-FITC/PI双染色流式细胞仪定量检测细胞凋亡率。结果:与模型对照组比较,含药血清组MDA含量显著降低(P<0.01)、SOD活力显著增强(P<0.01),细胞早期凋亡率、晚期凋亡及坏死率显著降低(P<0.01或P<0.05)。结论:复方五仁醇胶囊具有抗氧化和抑制肝细胞凋亡作用。

Studies on anti-oxidation and anti-apoptosis effects on hepatocytes by the medicated serum of Compound Wurenchun Capsula

Objective:To approach hepato-protective mechanism of Compound Wurenchun Capsula (CWC). Methods:The medicated serum was prepared after CWC ig to rat and the model of primary culture CCL4-induced hepatocyte injury was established. The contents of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in cell culture medium were measured after the CCL4-impaired hepatocytes were incubated by the medicated serum,and the apoptosis rate of these hepatocytes was detected by flow cytometer. Results:Compared with the model group,the content of MDA of the medicated serum group was decreased significantly(P<0.01),the activity of SOD was obviously improved(P<0.01),and the hepatocytes earlier apoptosis,advanced apoptosis and necrosis of hepatocytes was obviously decreased(P<0.01 or P<0.05). Conclusion:CWC has the effects of anti-oxidation and anti-apoptosis on hepatocytes.