短肠综合征病人血清氨基酸谱的测定

目的:用高效液相色谱法检测短肠综合征病人的血清氨基酸谱. 方法:共测定17例短肠综合征病人的血清氨基酸谱,并以15例正常人空腹血作为对照,将异硫氰酸苯酯(PITC)作为柱前衍生剂,用甲醇、乙腈和乙酸钠作为二元梯度洗脱,67 min完成分离. 结果:17例短肠综合征病人血清缬氨酸、丝氨酸、胱氨酸非常显著低于正常人(P<0.01),血清亮氨酸、异亮氨酸、赖氨酸、蛋氨酸、组氨酸显著低于正常人(P<0.05). 结论:用高效液相色谱法检测氨基酸稳定可靠,短肠综合征病人存在氨基酸特别是必需氨基酸的缺乏.

The measurement of serum amino acids in short bowel syndrome patients

Objective: To observe serum amino acids levels in patients with short bowel syndrome(SBS) by high performance liquid chromatography(HPLC). Methods: The serum samples of 17 SBS patients were collected, and 15 healthy volunteers were as the control group .PITC was used as pre-column derivative of HPLC. Two phases gradient elution was performed with methanol and acetic sodium for 67 minutes . Results: The serum levels of Val,Ser and Cys were significantly lower in SBS patients than that of healthy volunteers(P<0.01).The serum levels of Leu,Ile,Lys,Met and His in SBS patients were lower too (P<0.05). Conclusions: The HPLC method to detect the serum amino acids is steady and reliable. Most of amino acids, especially the essential amino acids,are greatly lacked in SBS patients.