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| 一种双链季铵盐消毒剂杀菌效果及其作用机理研究 | |
| 引用本文： | 赵进沛,张凤云,刘士敏,王忠海,李秀芹,张文勤.一种双链季铵盐消毒剂杀菌效果及其作用机理研究[J].中国消毒学杂志,2011,28(4). |
| 作者姓名： | 赵进沛 张凤云 刘士敏 王忠海 李秀芹 张文勤 |
| 作者单位： | 1. 北京军区疾病预防控制中心,北京,100042 2. 河北医科大学细胞生物教研室 3. 天津大学化学系 |
| 摘 要： | 目的研究一种双链季铵盐消毒剂的杀菌效果及作用机理。方法采用载体定量杀菌试验和扫描电镜观察的方法,对该季铵盐化合物杀灭金黄色葡萄球菌和大肠杆菌的效果及其超微结构进行了实验室观察。结果用100 mg/L该双链季铵盐消毒剂作用5 min,对载体(纸片)上金黄色葡萄球菌和大肠杆菌的杀灭率均达到100%。通过扫描电镜观察发现,经含量25 mg/L的该双链季铵盐消毒剂作用20 min的金黄色葡萄球菌菌体仍保有较完好的外部形态,但菌体表面粗糙,有少数菌体胞壁出现皱褶;可见到大肠杆菌表面出现凹陷和皱缩。经含量100 mg/L的该双链季铵盐消毒剂作用20 min的金黄色葡萄球菌的细胞壁有显著皱褶和破裂,表面有许多颗粒状物出现,形成小泡和小球;大肠杆菌菌体局部断裂破损。结论该双链季铵盐消毒剂在较低浓度具有快速杀灭细菌繁殖体的效果,其致死细菌可能与细胞质的凝集和胞内物质的渗漏有关。 |
| 关 键 词： | 双链季铵盐 杀菌效果 杀菌机理 细菌繁殖体 扫描电镜 |
STUDY ON THE GERMICIDAL EFFICACY AND MECHANISM OF A DOUBLE-CHAIN QUATERNARY AMMONIUM SALTS DISINFECTANT |
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| ZHAO Jin-pei,ZHANG Feng-yun,LIU Shi-min,WANG Zhong-hai,LI Xiu-qin,ZHANG Wen-qin.STUDY ON THE GERMICIDAL EFFICACY AND MECHANISM OF A DOUBLE-CHAIN QUATERNARY AMMONIUM SALTS DISINFECTANT[J].Chinese Journal of Disinfection,2011,28(4). | |
| Authors: | ZHAO Jin-pei ZHANG Feng-yun LIU Shi-min WANG Zhong-hai LI Xiu-qin ZHANG Wen-qin |
| Institution: | ZHAO Jin-pei,ZHANG Feng-yun1,LIU Shi-min,WANG Zhong-hai1,LI Xiu-qin,ZHANG Wen-qin2(Beijing Military Command Center of Disease Control and Prevention,Beijing 100042,1 Department of Cell Biology of Hebei Medical University,2 Department of Chemistry of Tianjin University,China) |
| Abstract: | Objective To study the germicidal efficacy and mechanism of a double-chain quaternary ammonium salts disinfectant.Methods Carrier quantitative gemicidal test and scanning electron microscope observation were used to observe the germicidal efficacy and the ultrastructure of Staphylococcus aureus and Escherichia coli.Results The killing rate were both 100% of S.aureus and E.coli by the disinfectant solution containing the double-chain quaternary ammonium salts 100 mg/L with 5 min contact time.Scanning electro... |
| Keywords: | double-chain quaternary ammonium salts germicidal efficacy germicidal mechanism vegetative forms of bacteria scanning electron microscope |
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