| 巢式PCR检测恶性淋巴瘤患儿骨髓人细小病毒B19DNA |
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| 引用本文： | 焦丽,陈靖,何君宏,刘新军,刘向萍,胡伟.巢式PCR检测恶性淋巴瘤患儿骨髓人细小病毒B19DNA[J].武警医学院学报,2003,12(1):44-45. |
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| 作者姓名： | 焦丽 陈靖 何君宏 刘新军 刘向萍 胡伟 |
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| 作者单位： | 武警陕西总队医院儿科,西安,710054 |
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| 摘 要： | 目的：用巢式聚合酶链反应（PCR）技术检测恶性淋巴瘤患儿骨髓中人细小病毒B19感染状态。方法：收集住院期间29例患有恶性淋巴瘤的患儿骨髓标本，对照组为非血液系统疾病的患儿骨髓标本26例行病例对照研究，用巢式PCR检测B19－DNA。结果：29例恶性淋巴瘤患儿骨髓中B19－DNA阳性9例，阳性率为31．0％，与对照组比较差异非常显（P＜0．01）；结论：提示恶性淋巴瘤患儿是B19－DNA的易感群体。化疗及免疫功能低下的淋巴瘤患儿发生B19感染易引起持续感染慢性贫血。
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| 关 键 词： | 巢式PCR 检测 恶性淋巴瘤 骨髓 细小病毒B19DNA 诊断 |
| 文章编号： | 1008-5041(2003)01-0044-02 |
| 修稿时间： | 2002年6月9日 |
Detection of human parvovirus B19 on bone marrow samples among children with malignant lymphoma by using nested-PCR |
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| JIAO Li,CHEN Jin,HE Jun-hong,LIU Xin-jun,LIU Xiang-ping,HU Wei..Detection of human parvovirus B19 on bone marrow samples among children with malignant lymphoma by using nested-PCR[J].Acta Academiae Medicinae CPAPF,2003,12(1):44-45. |
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| Authors: | JIAO Li CHEN Jin HE Jun-hong LIU Xin-jun LIU Xiang-ping HU Wei |
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| Abstract: | Objective:To detect B19-DNA on bone marrow samples among children with malignant lymphoma by using nested-polymerase china reaction(nested-PCR) technique that was the statea of parvovirue B19 infection in cildren with malignant lymphoma.Methods:We conducted a case-control study to investigate the infection of B19-DNA on bone marrow samples of 29 children with malignant lymphoma in our hospital and 26 cases of health children with nested-PCR techinique.Results:The positive rate of B19-DNA on bone marrow samples was 31 0% (9/29) in malignant lymphoma,significantly higher than in control group( P <0 01).Conclusion:The children with malignant lymphoma may be easy infected by human parvovirus B19.These results showed that children with malignant lymphoma who received chemotherapy and were immunosuppressed easily underwent parvovirus B19 infection,inducing chronic anemia. |
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| Keywords: | Children Malignant lymphoma Polymerase chain reaction Bone marrow samples Parvovirus B19 |
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