重组蛋白LHRH-PE40对卵巢癌细胞增殖与凋亡的影响

目的:研究重组蛋白LHRH-PE40对卵巢癌细胞增殖及凋亡的影响。方法:采用体外培养的人卵巢癌SK细胞进行细胞增殖抑制实验,在培养液中加入不同浓度的LHRH-PE40,于加药后1～4天用酶标分析仪分析LHRH-PE40对卵巢癌细胞增殖的影响。用免疫组化方法及流式细胞仪检测LHRH-PE40对卵巢癌SK细胞的增殖相关因子Ki-67、抗凋亡基因Bcl-2及细胞周期的影响。结果:LHRH-PE40可以有效抑制体外培养卵巢癌SK细胞的生长,呈时间和浓度的依赖性。最佳作用时间为加药后48～72小时。在药物浓度为1μg/ml的条件下,用药后癌细胞中的Ki-67(用药前73%,用药后25%)及Bcl-2(用药前92%,用药后43%)阳性细胞比率均下降。凋亡细胞比率增加(由用药前0.8%增加到用药后3.6%)。在0.5μg/ml浓度下,细胞的G0/G1期细胞比率增加;S期细胞比率增加,而G2/M期减少。结论:LHRH-PE40可杀灭卵巢癌细胞及促进卵巢癌细胞凋亡。

Effect of Recombinant Toxin LHRH-PE40 on Proliferation and Apoptosis of Ovarian Cancer Cell

Objective:To study the effects of recombinant protein LHRH-PE40 on ovarian cancer cell proliferation and apoptosis.Methods:Proliferation inhibition assay was taken in vitro cultured ovarian cancer SK cell.Different concentration of LHRH-PE40 was added into cell culture solution.The effects of LHRH-PE40 on ovarian cancer cell proliferation were analyzed by enzyme micro-plate reader 1-4 days after adding LHRH-PE40.The effects of LHRH-PE40 on proliferative factor Ki-67,anti-apoptosis gene Bcl-2 and cell cycle in ovarian cancer SK cells were detected by immunohistochemical staining and Cytometry.Results:LHRH-PE40 could inhibit the growth of in vitro cultured epithelial ovarian cancer SK cell,which presented time and concentration dependency.The best action time was 48-72 hours after administration of drugs.Under the concentration of medicine 1μg/ml,the positive cell rate of Ki-67(the rate before using medication was 73%,after was 25%) and Bcl-2(the rate before using medication was 92 %,after was 43%) was decreased after medication,and the ratio of apoptosis was increased(from 0.8% to 3.0%).Under 0.5 μg/ml concentration,the cell ratio of G0/G1 was also increased,in which the cell ratio of S phase was increased and the ratio of G2/M decreased.Conclusions:LHRH-PE40 can kill ovarian cancer cells and promote apoptosis of ovarian cancer cell.