HPLC法测定痰热清注射液中黄芩苷的含量

目的:建立以高效液相色谱法测定痰热清注射液中黄芩苷含量的方法。方法:色谱柱为CLC-ODS C18(150mm×6.0mm,5μm),流动相为甲醇-水(47∶53),流速为0.5mL·min-1,检测波长为280nm。结果:黄芩苷检测浓度在2.53～12.65μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.999 8);平均回收率为99.61%,RSD=0.35%(n=9)。结论:本法简便、快速,可用于痰热清注射液的质量控制。

Determination of Baicalin in Tanreqing Injection by HPLC

OBJECTIVE： To establish an HPLC method for the determination of Baicalin in Tanreqing injection. METHODS： The determination was performed by HPLC using an CLCODS C18 column（150 mm× 6.0 mm, 5 μm）, with mobile phase consisted of methanol- water（47 ： 53） at a flow rate of 0.5 mL · min^-1 and a detection wavelength of 280 nm. RESULTS： The calibration curve for Baicalin was linear in the concentration range of 2.53-12.65μg ·mL^-1（ r = 0.999 8）. The average recovery was 99.61% （RSD = 0.35%, n = 9） .CONCLUSION： The method is simple, rapid and applicable for the quality control of Tanreqing injection.