人乳头瘤病毒与P 53协同致膀胱移行细胞癌关系的研究

目的 研究人类乳头瘤病毒（ＨＰＶ）６、１１、１６和１８型及Ｐ５３与膀胱移行细胞癌的关系。方法 采用聚合酶链反应（ＰＣＲ）方法检测了７５例膀胱移行细胞癌组织中ＨＰＶ的感染，免疫组化ＳＰ法检测Ｐ５３蛋白表达情况。结果 膀胱移行细胞癌组织中ＨＰＶ６、１１、１６和１８的阳性率分别为６．７％（５／７５），５．３％（４／７５），３３．３％（２５／７５）和６．７％（５／７５）。低危型ＨＰＶ（６或１１）阳性率为９．３％（７／７５），高危型ＨＰＶ（１６或１８）阳性率为３４．７％（２６／７５）。同一膀胱癌组织中两种以上（包括两种）ＨＰＶ亚型感染８例，占１０．６％。ＨＰＶ６、１６和１８型之间感染阳性率在肿瘤有无转移组中差异显著（Ｐ〈０．０５），ＨＰＶ１６、１８的阳性率在肿瘤病理分级中差异有极显著性（Ｐ〈０．０１）。ＨＰＶ ＤＮＡ型别

The study on relation of human papillomavirus and P53 expression with bladder transitional cell carcinoma

Objective To study the relationship between the bladder transitional cell carcinoma and the human papillomavirus (HPV) types 6,11,16,18 and P53.Methods We used polymerase chain reaction (PCR) to test HPV type 6,11,16 and 18 infection from 75 cases of bladder cancer, and used immunohistochemisty method to detect P53 expression. Results The positive rates of HPV types 6,11,16 and 18 were 6.7 %, 5.3 %, 33.3 % and 6.7 %,respectively. The positive rate of low risk HPV type (6/11) was 9.3 %(7/75), and that of high risk HPV types (16/18) was 34.7 % (26/75). There were 8 cases with two or three HPV types mixed infection,accounting for 10.6 %, HPV types 6,11,16,18 in tumor metastasis group were significantly higher than that in non metastasis group. The positive rates of HPV 16,18 types in G3 were significantly higher than that in G1 and G2. HPV type 16 was the main type in bladder cancer. Positive rate of P53 expression was 44%. Conclusion These results implicated that the occurrence and development of bladder transitional cell carcinoma may be closely related to the high risk HPV type infection and P53 expression.