EVALUATION OF ANTIMICROBIAL AND CYTOTOXIC ACTIVITIES OF
PLANT EXTRACTS FROM SOUTHERN MINAS GERAIS CERRADO |
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Authors: | Juliana Moscardini Chavasco Bárbara Helena Muniz Prado E Feliphe Claudio Daniel Cerdeira Fabrício Damasceno Leandro Luiz Felipe Leomil Coelho Jéferson Junior da Silva Jorge Kleber Chavasco Amanda Latercia Tranches Dias |
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Institution: | Biomedical Science Institute, Microbiology and Immunology Department, Federal University of Alfenas, Alfenas, MG, Brazil |
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Abstract: | The antimicrobial activity of plant hidroethanolic extracts on bacteria
Gram positive, Gram negative, yeasts, Mycobacterium tuberculosis H37
and Mycobacterium bovis was evaluated by using the technique of Agar
diffusion and microdilution in broth. Among the extracts evaluated by Agar diffusion,
the extract of Bidens pilosa leaf presented the most expressive
average of haloes of growth inhibition to the microorganisms, followed by the extract
of B. pilosa flower, of Eugenia pyriformis'' leaf
and seed, of Plinia cauliflora leaf which statistically presented
the same average of haloes inhibitory formation on bacteria Gram positive, Gram
negative and yeasts. The extracts of Heliconia rostrata did not
present activity. Mycobacterium tuberculosis H37 and
Mycobacterium bovis (BCG) appeared resistant to all the extracts.
The susceptibility profile of Candida albicans and
Saccharomyces cerevisiae fungi were compared to one another and
to the Gram positive Bacillus subtilis, Enterococcus
faecalis and the Gram negative Salmonella typhimurium
bacteria (p > 0.05). The evaluation of cytotoxicity was carried
out on C6-36 larvae cells of the Aedes albopictus mosquito. The
extracts of stem and flower of Heliconia rostrata, leaf and stem of
Plinia cauliflora, seed of Anonna crassiflora
and stem, flower and root of B. pilosa did not present toxicity in
the analyzed concentrations. The highest rates of selectivity appeared in the
extracts of stem of A. crassiflora and flower of B.
pilosa to Staphylococcus aureus, presenting potential
for future studies about a new drug development. |
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Keywords: | Plant extracts Antimicrobial activity Mycobacterium tuberculosis Mycobacterium bovis Cytotoxicity |
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