| 高效液相色谱法测定小儿咳喘口服液中绿原酸的含量 |
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| 引用本文： | 邓云天,时东海. 高效液相色谱法测定小儿咳喘口服液中绿原酸的含量[J]. 中国中医药现代远程教育, 2012, 10(15): 165-F0003 |
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| 作者姓名： | 邓云天 时东海 |
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| 作者单位： | 1. 江苏省苏州君安药业有限公司,苏州,215129
2. 江苏省苏州雷奥生物科技有限公司,苏州,215104 |
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| 摘 要： | 目的 建立高效液相色谱法测定小儿咳喘口服液中绿原酸的含量.方法 Agela Promosil C18(250mm×4mm,5μm)分析柱,流动相为乙腈∶1%冰醋酸(11∶89),流速为1.0ml·min-1,检测波长为327nm.结果 绿原酸与其他组分分离良好.线性范围为0.1032～1.032μg,相关系数r=0.9998,平均加样回收率为98.93%(RSD= 0.4%).结论 本法简便、准确可靠,可用于该药的质量控制.
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| 关 键 词： | 高效液相色谱 小儿咳喘口服液 绿原酸 |
Determination of Chlorogenic Acid Pediatric Kechuan Oral Liquid By HPLC |
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| Deng yuntian , Shi donghai. Determination of Chlorogenic Acid Pediatric Kechuan Oral Liquid By HPLC[J]. Chinese Medicine Modern Distance Education of China, 2012, 10(15): 165-F0003 |
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| Authors: | Deng yuntian Shi donghai |
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| Affiliation: | 1 Jiangsu Suzou Junan Medical Corporation,Suzhou 215129,China; 2 Jiangsu Suzou Leiao Biological Science and Technology Ltd, Suzhou 215104 ,China) |
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| Abstract: | Objective To establish a HPLC method for the determination of pediatric Kechuan oral liquid on the content of chlorogenic acid. Methods Agela Promosil C18(250mm×4mm,5μm) column, the mobile phase consisted of acetonitrile -1% glacial acetic acid (11∶89), the flow rate was 1.0ml?min-1, the detection wavelength was 327 nm. Results Chlorogenic acid and other components were separated well. The linear range was 0.1032～1.032μg, the correlation coefficient r=0.9998, the average recovery rate was 98.93% (RSD= 0.4%). Conclusion This method is simple, accurate and reliable, and can be used for the quality control of drugs. |
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| Keywords: | High performance liquid chromatography Pediatric Kechuan oral liquid Chlorogenic acid |
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