一种检测血清β脂蛋白谱的新方法

目的 建立一种分离血清β脂蛋白(βLp)亚组分的新方法——二阶双梯度聚丙烯酰胺凝胶电泳(s-DGPAGE)法。方法 在不同浓度梯度凝胶中匹配一定的pH梯度，借助浓度梯度的分子筛作用和pH梯度的聚焦作用，促进βLp亚组分分离，通过凝胶成像系统和光密度扫描仪完成βLp谱的记录和量化分析。血清βLp的分离采用一阶DGPAGE。结果 s-DGPAGE法能将血清βLp分离出4种亚组分，分别命名为βLpl、βLp2、βLp3和βLp4，并可量化出各亚组分在βLp和血清脂蛋白整体中的相对含量。结论 s-DGPAGE法能捡出血清βLp的不均一组成，反映机体血清βLp的动态平衡状态，是一种简便实用的新方法。

A new method for detecting serum beta-lipoprotein electrophoretogram]

OBJECTIVE: To establish a new method for isolating serum beta-lipoprotein (betaLp) subfractions using second double gradient polyacrylamide gel electrophoresis (s-DGPAGE). METHODS: After the betaLp subfractions were isolated by mechanisms of molecular sieving and isoelectric focusing due to density and pH grade in the non-linear density gradient polyacrylamide gel, the gels were acquired with a gel imaging system, the electrophoretograms were quantitatively analyzed with optical density scanner. The isolation of serum betaLp was completed through first DGPAGE. RESULTS: s-DGPAGE separated the serum betaLp into four subfractions, designated respectively as betaLp1, betaLp2, betaLp3 and betaLp4, and their amounts in the betaLp and in the whole lipoproteins could be quantified. CONCLUSION: s-DGPAGE is a simple and practical method to show the heterogeneity of serum betaLp and the quantitative variation of its subfractions.