大鼠前脂肪细胞的原代培养分化

 目的 建立一种简便的大鼠前脂肪细胞的原代培养方法。 方法 无菌取成年Wistar大鼠的腹股沟纯脂肪颗粒，采用酶消化法进行原代培养。在倒置显微镜下观察细胞形态及生长情况，以油红O脂肪染色法进行原代培养细胞的鉴定。 结果 培养细胞的形态学观察显示，脂肪细胞贴壁后初为类圆形，3 d 后渐成梭形；7~8 d 细胞增殖明显，形状由多角梭形变为椭圆形，并开始积聚脂肪颗粒；10 d 后细胞呈椭圆形、圆形，胞内积聚大量脂肪颗粒。培养 10 d 的细胞经油红 O 染色后于普通光镜下观察，见细胞内出现红染颗粒，可以鉴定细胞分化为前脂肪细胞。 结论 成功建立了大鼠前脂肪细胞的原代培养方法，为脂肪内分泌学研究提供了制备适用细胞模型的手段。

Primary culture of rat preadipocyte

Objective To establish a new method for primary culture of rat preadipocytes. Methods Using enzyme-digesting method,fibroblast-like cells from the rat adipose tissues were cultured. The morphological changes of the cultured cells were observed under an inverted microscope and stained with oil red O to identify the primary cultured cells. Results Morphologically,the attached fat cells were round-like in shape initially,and then changed into spindle after 3 days. On day 7 to 8,the cells proliferated actively,and the polygonal/spindle cells turned into oval cells with fat particles accumulated inside. On day 10,the cells were oval of round in shape with a large amount of fat particles in the plasma. After being cultured for 10 days,red-stained particles were observed in the oil red O-stained cells under a light microscope,indicating that the cells had differentiated into preadipocytes. Conclution We have successfully established a method for primary culture of rat preadipocytes,which provides a basis to establish appropriate cell models for studies on adipose endocrinology.