| 酵母双杂交筛选人肝细胞文库戊肝病毒pORF3结合蛋白 |
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| 引用本文： | 康雁君,沈权,张文,华修国,冉雪琴,王嘉福. 酵母双杂交筛选人肝细胞文库戊肝病毒pORF3结合蛋白[J]. 中国人兽共患病杂志, 2010, 26(2): 111-114 |
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| 作者姓名： | 康雁君 沈权 张文 华修国 冉雪琴 王嘉福 |
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| 作者单位： | 贵州大学农业生物工程重点实验室;上海交通大学农业与生物学院人兽共患病实验室; |
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| 基金项目： | 上海市科攻关计划重点项目 |
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| 摘 要： | 目的筛选人肝细胞文库戊肝病毒pORF3结合蛋白,为HEVORF3蛋白的功能研究提供依据。方法构建pSOS-baitORF3诱饵重组载体,诱饵蛋白的自激活检测以及表达验证后,筛选cDNA文库中HEVORF3相互作用蛋白。结果经验证pSOS-baitORF3诱饵重组载体构建成功,诱饵蛋白的自激活检测以及表达验证均符合预期结果,共筛选出八种相互作用蛋白。结论筛选出的蛋白基本上为功能性蛋白,其中包括免疫信号通路激酶p110δ以及线粒体膜蛋白,和补体系统C3等,有进一步研究的价值。
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| 关 键 词： | 戊型肝炎病毒 作用蛋白 酵母双杂交 |
| 收稿时间： | 2010-02-20 |
Screening of the pORF3 binding protein of HEV in human liver cells by using yeast double hybridization system |
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| KANG Yan-jun,SHEN Quan,ZHANG Wen,HUA Xiu-guo,RAN Xue-qin,WANG Jia-fu. Screening of the pORF3 binding protein of HEV in human liver cells by using yeast double hybridization system[J]. Chinese Journal of Zoonoses, 2010, 26(2): 111-114 |
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| Authors: | KANG Yan-jun SHEN Quan ZHANG Wen HUA Xiu-guo RAN Xue-qin WANG Jia-fu |
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| Affiliation: | KANG Yan-jun1,SHEN Quan2,ZHANG Wen2,HUA Xiu-guo2,RAN Xue-qin1,WANG Jia-fu1(1.Key Laboratory for Agricultural Bioengineering of Guizhou Province,Guizhou University,Guiyang 550025,China,2.School of Agriculture , Biology,shanghai Jiaotong University,Shanghai 200240,China) |
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| Abstract: | HEV is a positive strand RNA virus containing 3 open reading frames(ORFs),while the function of its binding protein pORF3 is poorly understood.In our study,the interactive protein of the pORF3 from human liver cDNA library was screened by the SOS recruitment system,and the construction of the pSOS bait-ORF3 plasmid was identified.The expression of the pORF3 was analyzed by Western blotting,and the detection result of the self activation of bait protein was obtained as expected.Totally,8 putative interacting... |
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| Keywords: | hepatitis E virus interacting protein yeast double hybridization system |
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