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| 组胺对N-甲基-D-天门冬氨酸诱发的神经元损伤的改善作用 | |
| 引用本文： | 戴海斌,陈忠,黄育文,葛求富,张仲苗,魏尔清. 组胺对N-甲基-D-天门冬氨酸诱发的神经元损伤的改善作用[J]. 浙江大学学报(医学版), 2004, 33(3): 189-192 |
| 作者姓名： | 戴海斌 陈忠 黄育文 葛求富 张仲苗 魏尔清 |
| 作者单位： | 1. 浙江大学医学院,药理学教研室,浙江,杭州,310031;浙江大学医学院,附属第二医院,浙江,杭州,310009 2. 浙江大学医学院,药理学教研室,浙江,杭州,310031 3. 浙江大学医学院,附属第二医院,浙江,杭州,310009 |
| 基金项目： | 国家重点基础研究发展计划(973计划)，国家自然科学基金 |
| 摘 要： | 目的:在细胞水平探讨组胺对N-甲基-D-天门冬氨酸 (NMDA) 诱发的神经元损伤的改善作用及初步机制.方法:采用原代新生大鼠皮层培养的方法,以形态学和二苯基四氮唑溴盐染色为指标观察神经元的损伤和药物的改善作用.结果:组胺在10-4、10-6、10-7、10-8 mol/L浓度时能显著改善NMDA 50 μmol/L作用3 h引起的神经元死亡,并在10-4和10-7 mol/L浓度时分别呈现保护双峰.组胺10-7 mol/L的改善作用只被组胺H2受体西米替丁逆转,而组胺10-4 mol/L的改善作用只被H1受体吡拉明逆转.结论:组胺能减弱NMDA诱发的神经元兴奋性死亡,其保护作用在低浓度时可能与H2受体有关,在高浓度时可能与H1受体有关. |
| 关 键 词： | 组胺/药理学 N-甲基-D-天门冬氨酸 神经元/病理学 细胞,培养的 |
| 文章编号： | 1008-9292(2004)03-0189-04 |
| 修稿时间： | 2003-12-31 |
Reversing effect of histamine on neuron death induced by N-methyl-D-aspartate |
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| DAI Hai bin,CHEN Zhong,HUANG Yu wen,et al. Reversing effect of histamine on neuron death induced by N-methyl-D-aspartate[J]. Journal of Zhejiang University. Medical sciences, 2004, 33(3): 189-192 | |
| Authors: | DAI Hai bin CHEN Zhong HUANG Yu wen et al |
| Affiliation: | Department of Pharmacology, College of Medicine, Zhejiang University, Hangzhou 310031, China. |
| Abstract: | OBJECTIVE: To determine the effect of histamine on N-methyl-D-aspartate (NMDA) induced neuron death and to elucidate its mechanism. METHODS: The primary cortical cell culture was adopted. Neuron morphology and MTT assay were used to evaluate the drugs effects. RESULT: Histamine at doses of 10(-4) 10(-6) 10(-7) 10(-8) mol/L reversed the neuron death induced by NMDA (50 micromol/L) for 3 h. The protection of histamine peaked at doses of 10(-4) mol/L and 10(-7)mol/L. The effect of histamine of 10(-7) mol/L was reversed only by cimetidine an H(2)receptor antagonist. However, the effect of histamine of 10(-4) mol/L was reversed only by pyrilamine but not cimetidine. CONCLUSION: Histamine could reduce neuron death induced by NMDA; its protection at a low dose might be mediated by H(2)receptor, and at a high dose by H(1)receptor. |
| Keywords: | Histamine/pharmacol N-methyl-D-aspartate Neurons/pathol Cell cultured |
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