肝门部胆管癌神经浸润动物模型的建立

目的建立肝门部胆管癌神经浸润裸鼠模型。方法将培养的胆管癌细胞系QBC939细胞接种于裸鼠背部皮下,建立裸鼠背部皮下原代胆管细胞癌动物模型,取原代肿瘤组织接种于裸鼠腹腔近肝脏处,建立腹腔二代移植瘤模型,成瘤后行种植瘤组织解剖学和病理学观察。结果肝门部胆管癌背部皮下原代种植瘤成瘤率为100%（5/5）,未见神经浸润;腹腔二代移植瘤成瘤率为45%（9/20）,神经浸润率为22%（2/9）,神经浸润模型成功率为10%（2/20）。病理学观察显示为瘤细胞大小不一,形态多样,不规则;细胞分化程度低,胞浆减少,细胞核大,异形性明显,大小不等,可见核分裂像;无明显腺管样结构。结论肝门部胆管癌细胞具有较强的神经浸润性,应用QBC939细胞系建立肝门部胆管癌神经浸润裸鼠模型为研究肝门部胆管癌神经浸润方式及生物学特性创建了良好的实验平台。

Establishment of Animal Models of Hilar Cholangiocarcinoma with Perineural Invasion

Objective To establish perineural invasion xenograft model of hilar cholangiocarcinoma.Methods The cultured cells of cholangiocarcinoma cell line QBC939 were inoculated subcutaneously in the nude mice so as to establish primary subcutaneous model of cholangiocarcinoma.The primary tumor tissues were inoculated intraperitoneally around the liver in the nude mice so as to establish the second generation intraperitoneal xenograft model.The successful xenografted tumor tissues were obtained for anatomical and pathological examinations.Results The tumor formation rate of primary subcutaneous xenograft of hilar cholangiocarcinoma was 100%（5/5）,and no nerve infiltration was observed.The tumor formation rate of the second generation intraperitoneal xenograft was 45%（9/20）,and two mice（2/9,22%） manifested nerve infiltration.The rate of nerve infiltration was 10%（2/20）,and the tumor cells had different size and diversity,irregular shape,low differentiation,decreased cytoplasm and nucleus karyomegaly,visible atypical and fission phase,and no obvious gland tube structure by pathological examination.Conclusions Hilar cholangiocarcinoma cell has the particular features of perineural invasion,it is a good experiment platform for researching the mode and biological characteristics of perineural invasion of hilar cholangiocarcinoma by applicated QBC939 cell lines to establish the perineural invasion xenograft model of cholangiocarcinoma.