RhoA和Snail的表达与胃癌生物学行为的关系

目的检测RhoA和Snail在胃癌组织中的表达,以探讨它们与胃癌生物学行为的关系。方法采用免疫组织化学方法检测189例胃癌患者术后石蜡标本中RhoA和Snail蛋白的表达,分析它们的相关性以及与胃癌临床病理学特征和生存的关系。结果①RhoA和Snail在胃癌组织中的表达阳性率均分别明显高于其在癌旁组织（RhoA：P=0.008;Snail：P=0.000）和正常胃黏膜组织（RhoA：P=0.010;Snail：P=0.000）中的表达阳性率;RhoA在癌旁组织中的表达与其在正常胃黏膜组织中的表达差异无统计学意义（P=0.782）,而Snail在癌旁组织中的表达明显高于其在正常胃黏膜组织中的表达,差异有统计学意义（P=0.001）。②RhoA在胃癌组织中的阳性表达与胃癌的TNM分期和Lauren分型有关（P〈0.05）,而与胃癌的肿瘤直径、有无淋巴结转移及分化程度无关（P〉0.05）。Snail在胃癌组织中的阳性表达与胃癌肿瘤直径、有无淋巴结转移、分化程度、TNM分期及Lauren分型均有关（P〈0.05）。RhoA和Snail在胃癌组织中的阳性表达均与患者的性别和年龄无关（P〉0.05）。③胃癌组织中RhoA蛋白和Snail蛋白的表达呈显著正相关（rs=0.203,P=0.005）。④肿瘤TNM分期、RhoA及Snail表达和淋巴结转移均为胃癌术后患者的独立预后因素（P〈0.05）。结论 RhoA和Snail在胃癌组织中表达上调,它们可能共同参与了胃癌的发生、发展过程,且RhoA/Snail信号途径可能在胃癌的浸润和转移过程中发挥着重要作用。

Expressions of RhoA and Snail in Gastric Cancer Tissues and Relation Between These Expressions and Biological Behavior of Gastric Cancer

Objective To detect the expressions of RhoAand Snail in gastric cancer tissues,and explore the relationship of these expressions to the biological behavior of the gastric cancer.Methods The expressions of RhoA and Snail protein in the paraffin-embedded specimens of 189 gastric cancer patients were detected by immunohistochemical method.The relationships of their expressions to clinicopathologic features of gastric cancer or survival,and the relevance of RhoA expression and Snail expression were analyzed.Results ① The expressions of RhoA and Snail protein in the gastric cancer tissues were significantly higher than those in the paraneoplastic tissue（RhoA：P=0.008;Snail：P=0.000） and the normal gastric mucosa tissue（RhoA：P=0.010;Snail：P=0.000）;The expression of RhoA had no significant difference between the paraneoplastic tissue and the normal gastric mucosa tissue（P=0.782）,however,the expression of Snail in the paraneoplastic tissue was significantly higher than that in the normal gastric mucosa tissue（P=0.001）.② The expression of RhoA in the gastric cancer tissue was associated with TNM staging and Lauren type（P 0.05）,but it was not associated with tumor diameter,lymph node metastasis,or differentiation degree（P0.05）.The expression of Snail in the gastric cancer tissue was associated with tumor diameter,lymph node metastasis,differentiation degree,TNM staging,or Lauren type（P 0.05）.The expressions of RhoA and Snail in the gastric cancer tissue were not associated with patients’ gender and age（P0.05）.③ The expression of RhoA protein was significantly positi-vely correlated with Snail protein in the gastric cancer（r s =0.203,P=0.005）.④ The TNM staging of tumor,RhoAand Snail expressions,and lymph node metastasis were all the independent prognostic factors of postoperative gastric cancer patients（P0.05）.Conclusions RhoA and Snail proteins express in gastric cancer tissues,and involves in gastric carcinogenesis and the development process,and RhoA/Snail signaling pathway may play an important role in invasion and metastasis of gastric cancer.