人喉鳞癌细胞端粒长度与放射敏感性的关系探讨

 目的 探讨人喉鳞癌细胞端粒长度与放射敏感性的关系,以寻求能够预测肿瘤细胞内在放射敏感性的分子标志物. 方法 体外长期传代的人喉鳞癌细胞系Hep-2经0、2、4、8、12Gy剂量照射3次后的存活后代体外培养20代,以克隆形成实验测定其放射敏感性参数SF2,用Southern-blotting法测定其端粒长度(TRF). 结果 体外长期传代的Hep-2细胞系随着受照剂量的增加, 其放射存活后代的SF2逐渐升高(P<0.05),其TRF逐渐缩短(P<0.01);而且,SF2与TRF呈现明显的正相关关系(r=0.921, P<0.01). 结论 通过不同放射剂量处理体外长期传代的人喉鳞癌细胞系可获得不同放射敏感性的细胞存活后代,且放射存活后代的放射敏感性与其细胞端粒长度具有较好的负相关关系,提示端粒长度检测有望成为预测肿瘤细胞内在放射敏感性的分子标志物.

Correlation of Telomere Length and Radiosensitivity in Human Laryngeal Squamous Carcinoma Cells

Objective To investigate the correlation of telomere length and radiosensitivity in human laryngeal squamous carcinoma cells and to seek for the molecular marker that can be used to predict cellular intrinsic radiosensitivity. Methods The long-termly cultured human laryngeal squamous carcinoma cell line (Hep-2) was irradiated by the dose of 0, 2, 4, 8, 12Gy for three times and their survival progenies were obtained through subculturing 20 generations. Radiosensitivity index SF_2 was measured by clonogenic assay and telomere length (mean length of telomere restriction fragments, TRF) was examined by Southern-blotting. Results With the radiation doses' increasing, the SF_2 of survival progenies enhanced (P< 0.05) and the TRF prolonged (P< 0.01). Furthermore, the SF_2correlated positively with the TRF(r= 0.921, P< 0.01). Conclusion The survival progenies of Hep-2 cell line with different radiosensitivity can be obtained by irradiating with different doses. Furthermore, radiosensitivity correlates inversely with telomere length, which suggests that telomere length may be used as the predictor for cellular intrinsic radiosensitivity.