Genetic polymorphisms of OCT-1 confer susceptibility to severe progression of primary biliary cirrhosis in Japanese patients |
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Authors: | Yuki Ohishi Makoto Nakamuta Naoko Ishikawa Ohki Saitoh Hitomi Nakamura Yoshihiro Aiba Atsumasa Komori Kiyoshi Migita Hiroshi Yatsuhashi Nobuyoshi Fukushima Motoyuki Kohjima Tsuyoshi Yoshimoto Kunitaka Fukuizumi Makoto Ishibashi Takashi Nishino Ken Shirabe Akinobu Taketomi Yoshihiko Maehara Hiromi Ishibashi Minoru Nakamura |
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Affiliation: | 1. Department of Pharmacy, Clinical Research Institute, National Hospital Organization (NHO) Kyushu Medical Center, 1-8-1 Jigyouhama, Fukuoka, 810-8563, Japan 2. Department of Gastroenterology, Clinical Research Institute, National Hospital Organization (NHO) Kyushu Medical Center, 1-8-1 Jigyouhama, Fukuoka, 810-8563, Japan 3. Clinical Research Center, National Hospital Organization (NHO) Nagasaki Medical Center, Omura, 856-8562, Japan 4. Department of Hepatology, Nagasaki University Graduate School of Biomedical Sciences, 2-1001-1 Kubara, Omura, Nagasaki, 856-8562, Japan 5. Department of Pharmaceutical and Health Care Management, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, 814-0180, Japan 6. Department of Surgery and Science, Kyushu University Graduate School of Medical Sciences, Fukuoka, 812-8582, Japan 7. Headquarters of PBC Research in the NHO Study Group for Liver Disease in Japan (NHOSLJ), Nagasaki, Japan
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Abstract: | Background To identify the genetic factors involved in the pathogenesis of primary biliary cirrhosis (PBC), we focused on the organic cation transporter 1 (OCT1/SLC22A1), which is closely associated with phosphatidylcholine synthesis in hepatocytes. Methods We selected four (rs683369, rs2282143, rs622342 and rs1443844) OCT-1 single nucleotide polymorphisms (SNPs), and genotyped these SNPs using the TaqMan probe method in 275 Japanese PBC patients and 194 gender-matched, healthy volunteers as controls. Results The Chi-square test revealed that the rs683369 variant allele (G) was associated with insusceptibility to PBC development [P = 0.009, odds ratio (OR) 0.60, 95 % confidence interval (CI) 0.40–0.88] in an allele model, and that the rs683369 variant allele (G) was associated with jaundice-type progression in a minor allele dominant genotype model (P = 0.032, OR 3.10, 95 % CI 1.05–9.14). The OCT-1 rs2282143 variant (T) and rs622342 variant (C) were also associated with jaundice-type progression in a minor allele recessive genotype model (P = 0.0002, OR 10.58, 95 % CI 2.36–47.54, and P = 0.006, OR 7.84, 95 % CI 1.39–44.36, respectively). Furthermore, the association of OCT-1 rs683369 and rs622342 with susceptibility to jaundice-type progression was confirmed by a replication study with a distinct set of PBC patients who underwent liver transplantation. Conclusions The present study is the first report on the association of OCT-1 genetic polymorphisms with the overall development and jaundice-type progression of PBC. |
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