| DETECTION OF HUMAN TELOMERASE ACTIVITY BY TELOMERASE TRAP-ELISA ASSAY |
| |
| 引用本文： | 卫立辛,吴孟超,阎振林,沈锋,谢天培,钱其军,崔贞福. DETECTION OF HUMAN TELOMERASE ACTIVITY BY TELOMERASE TRAP-ELISA ASSAY[J]. 中国癌症研究, 1997, 9(4): 277-280. DOI: 10.1007/BF02974974 |
| |
| 作者姓名： | 卫立辛 吴孟超 阎振林 沈锋 谢天培 钱其军 崔贞福 |
| |
| 作者单位： | Wei Lixin Wu Mengchao Yan Zhenlin Shen Feng Xie Tianpei Qian Qijun Cui Zhenfu Shi Junxia Guo Yajun Tumor Immunology and Gene Therapy Center. Eastern Hospital of Hepatobiliary Surgery，Second Military Medical University，Shangha |
| |
| 摘 要： | ＤＥＴＥＣＴＩＯＮＯＦＨＵＭＡＮＴＥＬＯＭＥＲＡＳＥＡＣＴＩＶＩＴＹＢＹＴＥＬＯＭＥＲＡＳＥＴＲＡＰ—ＥＬＩＳＡＡＳＳＡＹＷｅｉＬｉｘｉｎ卫立辛ＷｕＭｅｎｇｃｈａｏ吴孟超ＹａｎＺｈｅｎｌｉｎ阎振林ＳｈｅｎＦｅｎｇ沈锋ＸｉｅＴｉａｎｐｅｉ谢天培Ｑｉａ...
|
Detection of human telomerase activity by telomerase TRAP — ELISA assay |
| |
| Lixin Wei,Mengchao Wu,Zhenlin Yan,Feng Shen,Tianpei Xie,Qijun Qian,Zhenfu Cui,Junxia Shi,Yajun Guo. Detection of human telomerase activity by telomerase TRAP — ELISA assay[J]. Chinese Journal of Cancer Research, 1997, 9(4): 277-280. DOI: 10.1007/BF02974974 |
| |
| Authors: | Lixin Wei Mengchao Wu Zhenlin Yan Feng Shen Tianpei Xie Qijun Qian Zhenfu Cui Junxia Shi Yajun Guo |
| |
| Affiliation: | (1) Tumor Immunology and Gene Therapy Center, Eastern Hospital of Hepatobiliary Surgery, Second Military Medical University, 200433 Shanghai |
| |
| Abstract: | Objective: Telomeric repeat amplification protocol (TRAP) is now a conventional assay for detecting telomerase activity. However, this method presents problems related to tedious quantifying, radioisotopic handing and the limited number of samples to be examined. In order to alleviate these inconveniences, we have developed and evaluated a novel telomerase TRAP-ELISA assay detecting human telomerase activity.Method: Telomerase TRAP-ELISA assay is a system based on the combination of PCR-ELISA with TRAP. Comparing with the conventional TRAP assay, we detected telomerase activity in 293 cell and negative controls (RNase-pretreated or heat-treated).Result: Telomerase activity in 293 cell is positive. The OD value of serial extracts from 10 , 102 , 103 and 104 cells assayed by telomerase PCR-ELISA depended on the number of 293 cells in assay. RNase-pretreated or heat-treated control was negative. Telomerase TRAP-ELISA detection yields results within one day and is handled without radioisotopes.Conclusion: Telomerase TRAP-ELISA assay is non-radioisotopic, fast and quantitative method for detecting human telomerase activity. Supported by grants from Chinese Natural Science Foundation |
| |
| Keywords: | Telomerase Detection ELISA |
| 本文献已被 CNKI SpringerLink 等数据库收录！ |
