LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling |
| |
Authors: | Brdicková Nadezda Brdicka Tomás Angelisová Pavla Horváth Ondrej Spicka Jiri Hilgert Ivan Paces Jan Simeoni Luca Kliche Stefanie Merten Camilla Schraven Burkhart Horejsí Václav |
| |
Institution: | Institute of Molecular Genetics, AS CR, Vídenská 1083, 142 20 Praha 4, Czech Republic. |
| |
Abstract: | Lymphocyte membrane rafts contain molecules critical for immunoreceptor signaling. Here, we report identification of a new raft-associated adaptor protein LIME (Lck-interacting molecule) expressed predominantly in T lymphocytes. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation. Thus, LIME appears to be involved in regulation of T cell activation by coreceptors. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|