脑表面大体摄影评价小鼠局灶性脑缺血后脑水肿变化

目的：建立脑表面大体摄影评价小鼠局灶性脑缺血后脑水肿变化的新方法。方法：采用大脑中动脉阻塞法诱导小鼠持续性局灶性脑缺血，于缺血后10、30min，1、3、6、12、24h取脑，用数码相机作脑顶面及侧面摄影；然后切成6片1mm厚的脑片进行摄影；最后，恒温烤干脑组织测定脑含水量。以MedBrain-2软件计算脑表面及脑片面积，评估脑水肿程度。结果：脑表面摄影测定面积的方法显示小鼠局灶性缺血后1h开始，缺血侧的脑面积明显增加，与脑含水量的结果一致。该方法测量脑水肿与脑含水量及脑片体积测量有较好的相关性。结论：脑表面大体摄影法是客观、定量评价局灶性脑缺血后脑水肿变化方法的一种补充，具有简便、敏感、不需前处理等优点。

Macrophotographic measurement of brain surface area for evaluating brain edema after focal cerebral ischemia in mice

OBJECTIVE: To establish a new macrophotographic measurement of brain surface area to evaluate brain edema after focal cerebral ischemia in mice. METHODS: Permanent focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) in mice. The brains were removed 10,30 min,1,3,6,12 and 24 h after MCAO, and photographed in dorsal and lateral views by a digital camera. Then, 6 coronal slices of 1 mm thick were cut and photographed. Finally, the water content of brain tissue was measured by heating at 110 degrees C for 24 h. The left and right hemisphere areas of the brains and the brain slices were analyzed and calculated by MedBrain 2 imaging analyzer to evaluate brain edema. RESULT: The macrophotographic measurement showed that the ischemic hemisphere areas significantly increased from 1 h after focal cerebral ischmia, which was similar to the measurement of water content. This measurement for brain edema correlated well with those of water content and brain slice volume. CONCLUSION: The macrophotographic measurement is an objective and quantitative method for evaluating brain edema after focal cerebral ischemia.