BI-1蛋白及其相关调控因子在非小细胞肺癌细胞系中的表达研究

目的 研究凋亡抑制因子BI-1与及其相关调控因子Bcl-2 、Bcl-XL在非小细胞肺癌细胞系中的表达差异，分析BI-1与Bcl-2、Bcl-XL的相互作用关系；方法 以9种非小细胞肺癌细胞系（NSCLC）为实验材料，采用RT-PCR和western blot 技术在cDNA和蛋白水平检测三种基因的表达；结果 发现BI-1在NSCLC中高表达，Bcl-2表达较低（在A549、PAa、GLC-82和SPC-1-A几乎检测不到），Bcl-XL表达较稳定，且明显高于Bcl-2；结论 在NSCLC细胞中，BI-1主要与Bcl-XL形成蛋白复合体作用于凋亡通路。

Study on the Expression of BI-1 and Relative Regulators in Human NSCLC Cell Lines

Objective To Investigate the expression of BI-1 and relative regulaters, Bcl-2 and Bcl-X_L in non-small cell lung cancer (NSCLC) cell lines,and analyze the interaction between BI-1 and Bcl-2, BI-1 and Bcl-X_L. Methods Using RT-PCR and Western blotting to detect the expression of BI-1, Bcl-2 and Bcl-X_L on the level of mRNA and protein in 9 NSCLC cell lines. Results The expression of BI-1 is the highest among the 3 genes. The expression of Bcl-2 is the lowest (nearly no expression in A549, PAa, GLC-82 and SPC-1-A). Bcl-X_L is stably expressed in 9 NSCLC cell lines, and obviously more than Bcl-2. Conclusion In 9 NSCLC cell lines, the protein complex of BI-1 and Bcl-X_L makes an important role in the process of apoptosis.