| 不同成熟时间对牛卵母细胞发育及去核效率的影响 |
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| 引用本文： | 施巧婷 黄俊成 等. 不同成熟时间对牛卵母细胞发育及去核效率的影响[J]. 国外畜牧学(草食家畜), 2001, 0(2): 1-3 |
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| 作者姓名： | 施巧婷 黄俊成 等 |
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| 作者单位： | [1]新疆大学生物系，乌鲁木齐830046 [2]新疆畜牧科学院农业部畜牧兽医生物技术重点开放实验室，乌鲁木齐830000 |
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| 摘 要： | 本文研究了不同成熟时间对牛卵母细胞的体外成熟、体外受精及后期的体外培养的影响和不同成熟时间的极体出现率。结果表明，牛卵母细胞在成熟16h时极体出现率达69.3%、分裂率为54.7％、囊胚率仅为6.25%；而成熟24h时极体出现率达76.9%、分裂率为70.0%、囊胚率为27.7%，差异均显著（P＜0.05）。成熟16h的卵母细胞刚刚排出第一极体，细胞核与极体非常近，此时去核率较高；而成熟24h的卵母细胞核已远离第一极体。
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| 关 键 词： | 牛 卵母细胞 体外成熟 第一极体 细胞发育 去核效率 成熟时间 |
| 文章编号： | 1003-6377(2001)02-0001-03 |
| 修稿时间： | 2001-01-18 |
Effect of Different Maturation Time on Bovine Oocyte Development and the Efficiency of De-nuclei |
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| SHI Qiao-ting ,HUANG Jun-cheng ,YANG Mei ,ZHANG Jia-xin ,WANG Li-hu ,LI Hui ,CUO Zhi-qin. Effect of Different Maturation Time on Bovine Oocyte Development and the Efficiency of De-nuclei[J]. Grass-Feeding Livestock, 2001, 0(2): 1-3 |
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| Authors: | SHI Qiao-ting HUANG Jun-cheng YANG Mei ZHANG Jia-xin WANG Li-hu LI Hui CUO Zhi-qin |
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| Affiliation: | SHI Qiao-ting 1,HUANG Jun-cheng 2,YANG Mei 2,ZHANG Jia-xin 3,WANG Li-hu 3,LI Hui 4,CUO Zhi-qin 2 |
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| Abstract: | Effect of different maturation time on bovine oocyte in vitro maturation,in vitro fertilization and developement of embryo and the first polar body extrude rate were studied.The results suggested that 69.3% of oocytes extruded their first polar body by 16h in vitro maturation.The cleavage rate and blastocyst rate were 54.7% and 6.25% after in vitro fertilization,whereas when in vitro maturation time reached 24h, the above data were 76.9%,70.0% and 27.7% respectively.The metaphase plate are relatively near to the first polar body when maturation time is 16h, the distance between metaphase plate and the first polar body became further when maturation time is 24h. |
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| Keywords: | bovine oocyte in vitro maturation the first polar body |
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