大豆异黄酮对老龄大鼠雌激素水平和子宫雌激素受体表达的影响

目的将白细胞介素2（IL-2）基因修饰树突状细胞（DC），观察其和对胰腺癌荷瘤裸鼠的治疗作用。方法将IL-2基因转染单个核细胞来源的DC后，用于激发自体细胞毒性T淋巴细胞（CTL），检测其对CTL的增殖和人胰腺癌PC3细胞杀伤性的影响：检测CTL对荷人胰腺癌PC3细胞肿瘤裸鼠肿瘤生长情况影响和对肿瘤细胞分裂指数和端粒酶活性的影响。结果IL-2基因修饰的DC细胞所激发的CTL对胰腺癌PC-3细胞有较强的抑制作用，并能抑制荷瘤裸鼠肿瘤细胞的分裂指数和端粒酶活性。结论IL-2基因修饰DC细胞激活的CTL抗PC-3肿瘤免疫作用明显增强，能明显抑制人胰腺癌PC3细胞种植瘤的生长。

Effect of Soy Isoflavones on Endogenous Estrogen Level and Uterine Estrogen Receptor Expression in Aged Rat

Objective To study the antitumor effect of cytotoxic T lymphocytes （CTL） induced by dendritic cells （DCs） transfected with IL-2 gene. Methods DCs collected from peripheral blood monocytes were transfected with IL-2 gene and CTL was induced by it. The proliferation of the CTL and its cytotoxic activity to PC3 was assessed. Nude mice with PC3 transplanted tumor were treated with CTL. The kinetics growth was recorded, RT-PCR-ELISA was used to determine the telomerase of transplanted tumor. Result CTL induced by DCs transfected with IL-2 gene can kill PC3 effectively, and it suppress the growth of experimental PC3 tumor and its telomerase. Conclusion DCs transfected with IL-2 gene activate the anti-tumor immune effect of CTL T lymphocytes and it may be a good immune therapy to malignant tumor.