广东省主要媒介害虫抗药性现状、对策研究及其应用

目的：研究广东省主要媒介害虫的抗药性现状及其防制对策。方法：生物测定法、酯酶滤纸法、ＰＣＲ技术及药激法。结果：经系统监测，广东省各监测点主要媒介对常用杀虫剂大部分都产生抗性，个别品系株已产生高度抗性。采用酯酶滤纸法快速检测家蝇抗药性及应用ＰＣＲ技术监测家蝇抗药性的研究，发现家蝇个体α－Ｎａ酯酶活性频率分布（ＥＦ５０值）与家蝇对ＤＤＶＰ、巴沙的敏感性（ＬＤ５０）呈一定的相关关系。ＤＤＶＰ、巴沙对家蝇

Studies on Insecticide Resistant Status and Countermeasure of Vectors of Public Health Importance and its Application in Guangdong Province

It was reported that the multiresistance appeared in several important vectors, some strains of vectors had developed high level resistance to some insecticides. The individual esterase activity filter paper test method and the PCR technical was used for monitoring resistance of Musca domestica . The results showed there were positive relativity between esterase activity and resistance ratio to DDVP or fenobcarb. The inhibitory frequency of DDVP or fenobcarb to esterase activity in the susceptible population was evidently higher than that of the DDVP or fenobcarb resistant strains. There was positive relativity between the amplificative frequency of specific resistant alleles gene in individual Musca domestica and resistance ratio to deltamethrin. There was a complete loss of flushing-out action for permethrin to resistant strain of cockroach, which had a high level resistance (RR>10 at LT 50 ). Control failure occurred in the restaurant with residual spread by deltamethrin SC at the doses of 30-40mg a.i/m 2 for cockroaches which had a high level resistance (RR>10 at LT 50 ). According to the resistant status in Guangdong province, measures to counteracting resistance were integrated management mainly with environmental management, development of resistant monitor method, development of new pesticides, new formulation, and new treatment method, and rational application of insecticides.The scientific research achievement was application of emergency disease vector control, development of health cities.