| 无创性肢体缺血预适应对大鼠心肌缺血/再灌损伤后纤溶因子的影响 |
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| 引用本文： | 李淑娟,吴艳娜,康毅,尹永强,高卫真,刘艳霞,娄建石.无创性肢体缺血预适应对大鼠心肌缺血/再灌损伤后纤溶因子的影响[J].中国药理学通报,2008,24(10). |
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| 作者姓名： | 李淑娟 吴艳娜 康毅 尹永强 高卫真 刘艳霞 娄建石 |
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| 作者单位： | 1. 天津医科大学药理学教研室,天津,300070;河北北方学院药理学教研室,河北,张家口,075000
2. 天津医科大学药理学教研室,天津,300070 |
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| 摘 要： | 目的观察无创性肢体缺血预适应(NLIP)对心脏缺血/再灌损伤后纤溶因子的影响,进一步探讨其对心脏的延迟保护作用。方法连续3d,每天1次,3个循环下肢无创性5min缺血,5min再灌建立NLIP模型。实验分3组:模型组(I/R);心脏缺血预适应组(CIP);NLIP组。再灌末取心肌组织,以四氮唑染色法测定心肌梗死面积(IS/AAR×100%)。取血,以发色底物法测定血浆中t-PA和PAI-1活性,以羟胺法测定血浆中SOD活性。结果与I/R组(44.5±8.1)%比较,CIP和NLIP能明显降低梗死面积(28.6%±9.5%和25.4%±8.7%,P<0.01);与I/R组t-PA(1.01±0.33)U·ml-1和PAI-1(27.11±0.63)AU·ml-1比较,CIP和NLIP均能明显对抗血t-PA活性的降低〔(1.98±0.46)和(1.89±0.44)U·ml-1,P<0.01〕和PAI-1活性的升高〔(25.42±0.56)和(22.01±0.45)AU·ml-1,P<0.01〕;与I/R组(300±49)NU·ml-1比较,CIP和NLIP均能明显提高再灌后SOD活性〔(366±72)和(388±67)NU·ml-1,P<0.05和P<0.01〕。结论NLIP对心肌缺血/再灌损伤具有保护作用,机制可能与其对纤溶系统的影响有关。
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| 关 键 词： | 无创伤 肢体 远端缺血预适应 t-PA PAI-1 SOD |
Influence of limb atraumatic ischemic preconditioning on fibrolysis factors in myocardial ischemia/reperfusion injury in rats |
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| LI Shu-juan,WU Yan-na,KANG Yi,YIN Yong-qiang,GAO Wei-zhen,LIU Yan-xia,LOU Jian-shi.Influence of limb atraumatic ischemic preconditioning on fibrolysis factors in myocardial ischemia/reperfusion injury in rats[J].Chinese Pharmacological Bulletin,2008,24(10). |
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| Authors: | LI Shu-juan WU Yan-na KANG Yi YIN Yong-qiang GAO Wei-zhen LIU Yan-xia LOU Jian-shi |
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| Institution: | LI Shu-juan1,2,WU Yan-na1,KANG Yi1,YIN Yong-qiang1,GAO Wei-zhen1,LIU Yan-xia1,LOU Jian-shi1(1.Dept of Pharmacology,Tianjin Medical University,Tianjin 300070,China,2.Dept of Pharmacology,Hebei North University,Zhangjiakou Hebei 075000,China) |
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| Abstract: | Aim To observe the effects of limb atraumatic ischemic preconditioning (NLIP) on the fibrolysis factors after myocardial ischemia-reperfusion (I/R) injury in rats.Methods RIP was performed in rats by a repeated three-cycle 5-min ischemia-reperfusion of hind limb everyday for three days.Three experimental groups were included:model,CIP and NLIP.The area at risk (AAR) and infarct area (IS) were determined using Trypan blue and triphenyl tetrazolium choride (TTC) staining respectively.The infarct size was defi... |
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| Keywords: | t-PA PAI-1 SOD |
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