巨细胞病毒对多发性骨髓瘤细胞系KM3细胞的感染及其对IL-6 mRNA表达的影响

目的　探讨人巨细胞病毒 (CMV)对多发性骨髓瘤细胞系KM3细胞的感染及其对细胞IL 6mRNA表达的影响。方法　以 10 0 ,10 ,1半数组织培养感染量 (TCID50 )滴度的CMV与KM3细胞共培养 ,RT PCR法检测细胞CMV即刻早期抗原基因 (IE)、甘油醛 3 磷酸脱氢酶基因 (GAPDH)及IL 6mRNA的表达 ,流式细胞术检测细胞CMVpp6 5抗原的表达 ,透射电镜检测细胞内CMV病毒颗粒。结果　CMV感染的KM3细胞可明显地表达IEmRNA ,同时该细胞IL 6mRNA水平明显升高 ;10 0 ,10TCID50滴度的CMV感染的KM3细胞CMVpp6 5抗原表达率分别为 (5 .5 8± 1.5 5 ) %、(3.75± 0 .85 ) % ,与对照组的 (1.5 8± 0 .33) %相比 ,差异有显著性 (P <0 .0 5 ) ;透射电镜下 ,10 0TCID50 滴度的CMV感染的KM3细胞内及胞膜表面可见到CMV病毒颗粒。结论　CMV可感染多发性骨髓瘤细胞系KM3细胞 ,并在其中活化复制 ;该病毒可提高KM3细胞IL 6mRNA的表达

Cytomegalovirus infection of myeloma cell line KM3 and its effect on IL-6 mRNA expression in the cells

OBJECTIVE: To investigate the infection of myeloma cell line KM3 by cytomegalovirus (CMV) as well as its effect on IL-6 mRNA in the cells. METHODS: RT-PCR assay was used to detect the mRNA expression of CMV immediate early gene (IE), glyceraldehyde phosphate dehydrogenase (GAPDH) and IL-6 of KM3 cells infected by 100-, 10-, 1-folds of median tissue culture infective dose (TCID(50)) of CMV. Flow cytometry was used to detect the expression of pp65 antigen. CMV particles were detected with electron microscope. RESULTS: KM3 cells infected by CMV could express IE mRNA as compared with the uninfected cells. CMV pp65 antigen positive cells were (5.58 +/- 1.55)% and (3.75 +/- 0.85)% respectively when infected by 100 and 10 TCID(50) of CMV, which were significantly higher than that of control group (P < 0.05). CMV particles could be seen in the infected cells. Expression of IL-6 mRNA was increased after the infection. CONCLUSION: CMV can infect myeloma cell line KM3 and replicate in the cells. CMV can increase the expression of IL-6 mRNA.