结直肠癌组织中硫氧还蛋白-1与硫氧还蛋白互作蛋白的表达及其临床意义

目的　探讨硫氧还蛋白-1（TRX-1）以及硫氧还蛋白互作蛋白（TXNIP）在结直肠癌组织中的表达及其临床意义。方法　选取2019年1~12月河北北方学院附属第一医院收治的结直肠癌患者72例，另选取同时期在该院接受治疗的结直肠息肉患者40例作为对照组。采用免疫组化法检测手术切除的病理标本中TRX-1和TXNIP的表达情况，分析结直肠癌组织中TRX-1和TXNIP的表达和临床病理特征的关系，分析结直肠癌组织中TRX-1和TXNIP表达的相关性，接受者工作特征曲线（ROC）分析TRX-1和TXNIP对结直肠癌的诊断价值。结果　结直肠癌组织中TRX-1的阳性表达率为66.67%，高于结直肠息肉组织中的25.00%，差异有统计学意义（χ2=17.880，P=0.000），结直肠癌组织中TXNIP的阳性表达率为30.56%，低于结直肠息肉组织中的77.50%，差异有统计学意义（χ2=22.733，P=0.000）。结直肠癌组织中TRX-1的表达与TNM分期、淋巴结转移、肿瘤大小以及分化程度有关（χ2=4.114~4.938，P=0.026~0.043）；结直肠癌组织中TXNIP的表达与TNM分期、淋巴结转移有关（χ2=4.675，5.531，P=0.019，0.031）。结直肠癌组织中TRX-1和TXNIP的表达呈负相关（r=-0.298，P=0.011）。TRX-1和TXNIP对结直肠癌有一定的诊断价值，曲线下面积分别为0.708和0.735，而二者联合可进一步提高诊断价值，曲线下面积为0.792。结论　TRX-1在结直肠癌组织中异常高表达，TXNIP异常低表达，二者的表达水平均与部分临床病理参数有关，且对结直肠癌均有一定的诊断价值。

Expression and Clinical Significance of Thioredoxin-1 and Thioredoxin Interacting Protein in Colorectal Cancer

Objective　To investigate the expression and clinical significance of thioredoxin-1 (TRX-1) and thioredoxin interaction protein (TXNIP) in colorectal cancer. Methods　A total of 72 patients with colorectal cancer who were admitted to the First Affiliated Hospital of Hebei North University from January 2019 to December 2019 were selected, and 40 patients with colorectal polyps who were received treatment in the hospital during the same period were selected as controls group. Immunohistochemical method was used to detect the expression of TRX-1 and TXNIP in surgically resected pathological specimens. The relationship between the expression of TRX-1 and TXNIP in colorectal cancer tissues and clinicopathological characteristics were analyzed, and the correlation between the expression of TRX-1 and TXNIP in colorectal cancer were analyzed. Receiver operating characteristic curve (ROC) was used to analyze the diagnostic value of TRX-1 and TXNIP for colorectal cancer. Results　The positive expression rate of TRX-1 in colorectal cancer tissue was 66.67%, which was higher than 25.00% in colorectal polyp tissue, the difference was statistically significant(χ2=17.880, P=0.000).The positive expression rate of TXNIP in colorectal cancer tissue was 30.56%, which was lower than 77.50% in colorectal polyp tissue, the difference was statistically significant(χ2=22.733, P=0.000). The expression of TRX-1 in colorectal cancer tissue was related to TNM stage, lymph node metastasis, tumor size and degree of differentiation (χ2=4.114~4.938, P=0.026~0.043), and the expression of TXNIP in colorectal cancer tissue was related to TNM stage and lymph node metastasis (χ2=4.675, 5.531, P=0.019, 0.031). The expression of TRX-1 and TXNIP in colorectal cancer tissue was negatively correlated (r=-0.298,P=0.011). TRX-1 and TXNIP had certain diagnostic value for colorectal cancer, and the area under the curve was 0.708 and 0.735, respectively. The combination of the two could further improve the diagnostic value, the area under the curve was 0.792. Conclusion　TRX-1 was abnormally highly expressed in colorectal cancer tissues, and TXNIP was abnormally lowly expressed. The expression levels of both were related to some clinicopathological parameters, and both have certain diagnostic value for colorectal cancer.