Analysis of Ergot Alkaloids |
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Authors: | Colin Crews |
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Affiliation: | Fera Science Ltd., Sand Hutton, North Yorks YO41 1LZ, UK; E-Mail: ; Tel.: +44-1904-462-549; Fax: +44-1904-462-111 |
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Abstract: | The principles and application of established and newer methods for the quantitative and semi-quantitative determination of ergot alkaloids in food, feed, plant materials and animal tissues are reviewed. The techniques of sampling, extraction, clean-up, detection, quantification and validation are described. The major procedures for ergot alkaloid analysis comprise liquid chromatography with tandem mass spectrometry (LC-MS/MS) and liquid chromatography with fluorescence detection (LC-FLD). Other methods based on immunoassays are under development and variations of these and minor techniques are available for specific purposes. |
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Keywords: | ergot LSD ergotamine lysergic acid ergovaline mycotoxins |
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