食管癌患者血清神经元特异性烯醇化酶与长链非编码RNA ITGA9-AS1水平联合检测的实验诊断价值研究

目的　探究食管癌(esophagus cancer, EC)患者血清神经元特异性烯醇化酶(neuronspecific enolase, NSE)与长链非编码RNA(long non-coding RNA, lncRNA) ITGA9-AS1水平联合检测的实验诊断价值。方法　选择2018年5月~2019年7月兵器工业五二一医院收治的112例EC可疑病变患者为研究对象，经病理学检查确诊67例EC患者为EC组，45例食管良性病变为食管良性病变组。采用电化学发光免疫分析仪检测NSE水平，采用实时荧光定量PCR(quantitative Real-time PCR, qRT-PCR)检测血清lncRNA ITGA9-AS1的水平。Pearson相关分析分析血清NSE，lncRNA ITGA9-AS1水平与EC患者病理分期、肿瘤浸润深度的相关性。结果　EC组患者血清NSE（22.71±3.03μg/L vs 2.98±0.22μg/L）和lncRNA ITGA9-AS1（0.88±0.21 vs 0.64±0.14）的表达水平高于食管良性病变组，差异具有统计学意义 (t=13.33，6.41，均P=0.00)。III期EC患者血清NSE和lncRNA ITGA9-AS1水平显著高于I期和II期，差异具有统计学意义(P＜0.01)，另外肿瘤浸润深度T4血清NSE和lncRNA ITGA9-AS1水平显著高于T1，差异具有统计学意义(P＜0.01)。Pearson相关性分析结果显示，血清NSE，lncRNA ITGA9-AS1水平与EC患者病理分期、肿瘤浸润深度呈显著正相关(r=0.142，P=0.005和r=0.171，P=0.013)。血清NSE，lncRNA ITGA9-AS1诊断敏感度均较低，但均具有较高的特异度。联合检测在EC中有较高的敏感度和特异度，分别为82.1%和83.2%。结论　血清NSE和lncRNA ITGA9-AS1定量检测可用于EC的诊断，联合检测可提高诊断的敏感度。

Experimental Diagnostic Value of Combined Detection of SerumNeuron-Specific Enolase and Long Non-Coding RNA ITGA9-AS1 inPatients with Esophageal Cancer

Objective　To explore the diagnostic value of combined detection of serum neuronspecific enolase (NSE) and longnon-coding RNA(lncRNA) ITGA9-AS1 in patients with esophagus cancer (EC). Methods　A total of 112 patients withsuspected EC lesions admitted to 521 Hospital of Norinco Group from May 2018 to July 2019 were selected as the study subjects.67 patients with EC confirmed by pathological examination were classified as EC group, and 45 patients with benign esophageallesions were classified as esophageal benign lesions group. The level of NSE was detected by electrochemiluminescenceimmunoanalyzer, and the level of serum lncRNA ITGA9-AS1 was detected by quantitative real-time PCR (qRT-PCR). Results　Theexpression levels of serum NSE(22.71±3.03μg/L vs 2.98±0.22μg/L) and lncRNA ITGA9-AS1 (0.88±0.21 vs 0.64±0.14) inEC group were higher than those in esophageal benign lesions group, and the difference was statistically significant (t=13.33,6.41, all P=0.00). The serum levels of NSE and lncRNA ITGA9-AS1 in stage III EC patients were significantly higher than thosein stage I and II, with statistical significance (P<0.01). In addition, the serum levels of NSE and lncRNA ITGA9-AS1 in T4patients with tumor invasion depth were significantly higher than those in T1, with statistical significance (P <0.01). Pearsoncorrelation analysis showed that serum NSE, lncRNA ITGA9-AS1 levels were significantly positively correlated withpathological stage and tumor invasion depth in EC patients (r=0.142, P=0.005 and r=0.171, P=0.013). Serum NSE and lncRNA ITGA9-AS1 had low diagnostic sensitivity, but both had high specificity and sensitivity. The sensitivity and specificity of thecombined test in EC were 82.1% and 83.2%, respectively. Conclusion　Quantitative detection of serum NSE and lncRNAITGA9-AS1 could be used in the diagnosis of EC, and the combined detection can improve the sensitivity of the diagnosis.