Identification of monoclinic calcium pyrophosphate dihydrate and hydroxyapatite in human sclera using Raman microspectroscopy |
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| Authors: | Ko-Hua Chen Mei-Jane Li Wen-Ting Cheng Tonci Balic-Zunic Shan-Yang Lin |
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| Affiliation: | Department of Ophthalmology, Taipei Veterans General Hospital and National Yang-Ming University, Taipei, Taiwan;, Medical Engineering Section, National Health Research Institute, Taipei, Taiwan;, Department of Medical Research &Education, Taipei Veterans General Hospital, Taipei, Taiwan;and Geological Institute, University of Copenhagen, Kobenhavn, Denmark |
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| Abstract: | Raman microspectroscopy was first used to determine the composition of a calcified plaque located at the pterygium-excision site of a 51-year-old female patient's left nasal sclera after surgery. It was unexpectedly found that the Raman spectrum of the calcified sample at 1149, 1108, 1049, 756, 517, 376 and 352/cm was similar to the Raman spectrum of monoclinic form of calcium pyrophosphate dihydrate (CPPD) crystal, but differed from the Raman spectrum of triclinic form of CPPD. An additional peak at 958/cm was also observed in the Raman spectrum of the calcified plaque, which was identical to the characteristic peak at 958/cm of hydroxyapatite (HA). This is the first study to report the spectral biodiagnosis of both monoclinic CPPD and HA co-deposited in the calcified plaque of a patient with sclera dystrophic calcification using Raman microspectroscopy. |
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| Keywords: | calcium pyrophosphate dihydrate (CPPD) dystrophic calcification hydroxyapatite (HA) Raman microspectroscopy sclera |
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