子痫前期患者血清和胎盘的Fas与FasL的变化及意义

目的:比较子痫前期(PE)患者与正常孕妇血清和胎盘Fas/FasL的表达,探讨Fas/FasL系统的表达与子痫前期的关系.方法:选择40例子痫前期患者和39例正常孕妇作为研究对象,采用ELISA方法测定血清sFas和sFasL水平;采用Western Blot检测胎盘Fas和FasL的蛋白含量.结果:子痫前期组血清sFas水平(2.11±0.95)mg/L高于正常对照组(1.57±0.60)mg/L,差异有显著性(P<0.05); 子痫前期组血清sFasL水平(4.43±1.90)μg/L也显著高于正常对照组(3.48±1.53)μg/L,P<0.01;两组胎盘中均测得Fas及FasL蛋白表达,但二者差异均无显著性(P>0.05).结论:Fas和FasL的改变与PE相关,血清游离Fas和FasL增高对子痫前期的发生和发展可能起着重要作用,Fas/FasL的异常表达导致妊娠免疫耐受不当可能是PE发病的重要机制之一.

Expression of Fas and FasL in serum and placenta of preeclamptic pregnancy and its significance

OBJECTIVE: To observe the serum and placental levels of FAS and FASL in preeclampsia (PE) and to study its relationship with the disease. METHODS: Forty women with preeclampsia and 39 healthy pregnant women were recruited and samples of serum and placentas were collected. The expression of Fas and FasL in placentas was detected with Western blot and the concentration of soluble Fas and FasL in serum was detected with ELISA method. RESULT: Serum levels of soluble Fas in PE group were significantly higher than those of healthy pregnant women (2.11+/-0.95 mg/L compared with 1.57+/-0.60 mg/L, P<0.05), and serum levels of soluble FasL in PE group were also significantly higher than those in controls (4.43+/-1.90 g/L compared with 3.48+/-1.53 g/L, P<0.01). There were no significant differences in Fas and FasL levels in placentas between PE group and healthy pregnant women (P>0.05 for both). CONCLUSION: The elevated serum Fas and FasL levels are closely associated with preeclampsia, which may play an important role in the pathogenesis of the disease.