Role of the spleen in peripheral memory B-cell homeostasis in patients with autoimmune thrombocytopenia purpura |
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Authors: | Martinez-Gamboa Lorena Mei Henrik Loddenkemper Christoph Ballmer Boris Hansen Arne Lipsky Peter E Emmerich Florian Radbruch Andreas Salama Abdulgabar D?rner Thomas |
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Affiliation: | Charité Center 14 for Tumor Medicine, Institute for Transfusion Medicine and Immune Hematology, Charité University Hospital, Berlin, Germany. |
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Abstract: | The effect of splenectomy on circulating memory B cells in autoimmune thrombocytopenia purpura (AITP) patients has not yet been addressed. We therefore analyzed the distribution and phenotypic characteristics of B-cell subsets in non-splenectomized and splenectomized AITP patients and controls, as well as CD95 expression after B cell activation. Decreased frequencies of memory B cells in splenectomized individuals were observed, with a rapid decline of CD27+IgD+ and a slower decrease of CD27+IgD- and CD27-/IgD- cells. Similar results were noted following splenectomy in healthy donors (HD). CD95+ B cells were substantially increased in all subsets in patients with active AITP, indicating their enhanced activation status. After splenectomy, the percentage of CD95+ B cells were further increased in the CD27+IgD- post-switch memory population in AITP, but not in HD. CD95+CD27+ memory B cells largely reside in the region in the human spleen analogous to the murine marginal zone. Thus, the spleen plays a fundamental role in controlling peripheral memory B cell homeostasis in both AITP and HD and regulates activated CD95+ B cells in patients with AITP. |
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