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减少分枝杆菌培养污染的方法学研究
引用本文:吴龙章,黄洁玲,谢敏娜,蔡杏珊,陈素颖,汪福康.减少分枝杆菌培养污染的方法学研究[J].中国防痨通讯,2006,28(6):398-400.
作者姓名:吴龙章  黄洁玲  谢敏娜  蔡杏珊  陈素颖  汪福康
作者单位:广州市胸科医院 广州 510095;
摘    要:目的减少分枝杆菌培养过程中出现的污染问题,以提高分枝杆菌的检出率。方法对污染标本进行细菌学培养,了解某时期污染标本的微生物分布情况,并根据分离出的优势污染株有针对性地在该时期对送检标本改用酸性前处理剂或加大氢氧化钠的浓度进行样品的前处理,且与常规的前处理方法进行对比研究。结果对主要由革兰阴性肠道科细菌引起的污染,改用5%硫酸做前处理剂,以及对由革兰阳性菌(包括酵母样菌属等)引起的污染,加大氢氧化钠的浓度,可使培养过程中出现的污染率由6.5%下降至2.4%。结论对某时期的污染标本进行细菌学培养与分析,并有针对性地在此阶段对样品改用酸性前处理剂或加大氢氧化钠的浓度,可以有效地减少在培养分枝杆菌过程中出现的污染情况。

关 键 词:分枝杆菌  结核  设备污染  培养基  
修稿时间:2005年3月24日

A methodological research on the way to reduce the pollution in Mycobacterium culture
Wu Longzhang,Huang Jieling,Xie Minna,et al..A methodological research on the way to reduce the pollution in Mycobacterium culture[J].The Journal of The Chinese Antituberculosis Association,2006,28(6):398-400.
Authors:Wu Longzhang  Huang Jieling  Xie Minna  
Institution:Department of Clinical Laboratory,Guangzhou Chest Hospital,Guangzhou 510095,China.
Abstract:Objective To reduce the pollution in mycobacterium culture and increase mycobacterium detective rate. Methods Culture was made to the polluted specimens to determine the distribution of polluted microorganism species in a certain period,and predominant bacterial species were analysed.Accoding to different species of predominant bacterium,different predisposal ways(acid pretreated agent or increasing the concentration of sodium hydroxide) were employed to pretreat samples before mycobacterium culture.This methodological improvement was compared with normal predisposal methods. Results Pretreating sample with 5% sulfuric acid when it was predominantly polluted by gram negative intestinal bacterium or with high density sodium hydroxide when it was predominantly polluted by gram positive bacterium(including yeastlike bacterium) may decrease pollution risk from 6.5% to 2.4%. Conclusion Selectively applying predisposal Methods according to different species of predominant polluted microorganism in a certain period may effectively reduce mycobacterium mycobacterium culture.
Keywords:Mycobacterium tuberculosis  Equipment contamination  Culture media
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