层流低切应力刺激对人脐静脉血管内皮细胞Toll样受体mRNA表达的影响

目的用RT-PCR和Northern杂交技术,评价层流低切应力刺激对人脐静脉血管内皮细胞Toll样受体(TLR-4)信号受体表达的影响。方法用未刺激和经42μN/cm2处理1 h的脐静脉血管内皮细胞提取总RNA,采用RT-PCR和Northern杂交技术观察切应力刺激1 h人脐静脉血管内皮细胞TLR-2和TLR-4 mRNA的表达情况。结果RT-PCR和Northern杂交均显示,层流低切应力刺激1 h后血管内皮细胞TLR-4表达增强,TLR-2几乎无变化。结论层流低切应力可引起人脐静脉内皮细胞TLR-4mRNA表达增加,提示炎症信号受体TLR-4可能介导层流切应力诱导的血管内皮细胞某些效应基因的表达。

The Changes of Toll-like Receptor mRNA Expression in Human Umbilical Vein Endothelial Cells after Lower Laminar Shear Stress

Objective By using the technology of RT-PCR and Northern hybridization,to assess the expression of TLR-2 and TLR-4 in human umbilical vein endothelial cells(HUVECs) after lower laminar shear stress.Methods Total RNA was isolated from HUVECs untreated and treated with 42 μN/cm² for 1 h.The technology of RT-PCR and Northern hybridization were used to determine the change of TLR-2 and TLR-4 mRNAs expression after 1 hour stimulation of laminar shear stress.Results The results of RT-PCR and Northern hybridization showed that the expression of TLR-4 in HUVECs was increased after 1 h shear stress.There was no change for TLR-2 after stimulation.Conclusion Lower laminar shear stress can induce TLR-4 mRNA expression.The experiment suggests that the inflammatory signal receptor TLR-4 would probably be involved in flow shear stress-induced effective gene expression in human vascular endothelial cells.