RP-HPLC法测定神曲胃痛胶囊中大黄素、大黄酚的含量

目的:建立以反相高效液相色谱法测定神曲胃痛胶囊中大黄素、大黄酚含量的方法。方法:色谱柱为Phenomenex BDS-C18(250mm×4.6mm,5μm),流动相为乙腈-水-冰醋酸(55∶45∶0.5),流速为1.0mL.min-1,检测波长为254nm。结果:大黄素、大黄酚的检测浓度分别在2.188~54.700、4.312~107.800μg.mL-1范围内与各自峰面积积分值呈良好的线性关系(r均为0.9999);二者平均回收率分别为96.52%、98.45%,RSD分别为0.59%、0.43%。结论:本方法简便、可靠、重现性好,可用于神曲胃痛胶囊的质量控制。

Determination of Emodin and Chrysophanol in Shenqu Weitong Capsules by RP-HPLC

OBJECTIVE： To determine the contents of Emodin and Chrysophanol in Shenqu weitong capsule by RPHPLC. METHODS： The samples were separated on Phenomenex BDS- C18（250 mm × 4.6 mm, 5μm） .The mobile phase consisted of acetonitrile-water-glacial acetic acid （55 ： 45 ： 0.5） at a flow rate of 1.0 mL · min^-1. The detection wavelength was set at 254 nm. RESULTS： The linear range for Emodin was 2.188-54. 700μg · mL^-1（r = 0.999 9） and 4.312-107. 800 μg · mL^-1 for Chrysophanol （r = 0.999 9） . The average recovery of Emodin was 96.52% （RSD = 0.59%） versus 98.4,5% （RSD = 0.43% ）of Chrysophanol. CONCLUSION： This method is convenient, accurate, reproducible and applicable for the quality contro; of Shenqu weitong capsule.