银杏叶提取物EGb761对大鼠脊髓损伤后神经损伤恢复的影响

目的研究银杏叶提取物EGb761对大鼠脊髓损伤后神经损伤恢复的作用。方法 SD大鼠120只,随机分为假手术组、损伤对照组、甲基强的松龙(MP)治疗组和EGb761治疗组,每组30只。用改良Allen法建立脊髓损伤的动物模型。采用斜板试验法和BBB行为学评分观察大鼠脊髓损伤后神经功能的恢复情况;HE染色观察脊髓大体组织结构变化;采用酶联免疫吸附试验(ELISA)法测定脊髓组织中IL-10的含量。结果与损伤对照组比较,MP治疗组及EGb761治疗组大鼠术后7d和14 d临界角度显著增加、BBB评分显著升高、大鼠脊髓组织中IL-10的含量显著增高。HE染色显示,损伤对照组脊髓组织发生出血、水肿、坏死、轴突脱髓鞘改变以及炎症细胞浸润和胶质细胞反应,MP治疗组及EGb761治疗组术后7 d脊髓组织水肿、炎症反应减轻,神经元变性坏死不明显。结论 EGb761能减轻受伤脊髓的继发性损伤,对大鼠脊髓损伤后神经损伤恢复具有促进作用。

Effect of Ginkgo biloba extract EGb761 on healing of nerve injury after spinal cord injury in rats

Objective To investigate the effect of Ginkgo biloba extract EGb761 on the healing of nerve injury after spinal cord injury in rats.Methods 120 SD rats were randomly divided into sham group,injury control group,methylprednisolone(MP) treated group and EGb761 treated group,30 rats for each group.The spinal cord injury model was established by modified Allen method.The recovery of neurological function after SCI in rats was observed with inclined plane test and BBB score.Pathological changes of spinal cord were observed through HE staining.IL-10 in spinal cord was conducted by ELISA.Results Compared to the injury control group,the critical angle,BBB score and IL-10 increased obviously in MP and EGb761 treated groups in the seventh and the fourteenth day after spinal cord injury.HE staining showed that there were hemorrhage,edema,necrosis,axonal demyelination,infiltration of inflammatory cells and astroglial responses in spinal cord in injury control group.In contrast,edema,inflammatory reaction,neuronal degeneration and necrosis were much less in MP and EGb761 treated groups in the seventh day after spinal cord injury.Conclusion EGb761 can alleviate the secondary spinal cord injury and promote the recovery of nerve injury after spinal cord injury in rats.