HIV和HCV感染者重叠感染HGV对病毒复制的影响

目的 :了解人免疫缺陷病毒 (HIV)感染者和丙型肝炎病毒 (HCV)感染者重叠感染庚型肝炎病毒 (HGV)的情况 ,探讨重叠感染病毒在体内的相互作用机理。方法 :用定量 PCR测定 HIV和 HCV感染者血浆中病毒载量 ,同时用 RT- PCR检测这些患者 HGV的感染情况 ,并对部分 HGV阳性者进行序列测定。结果 :317例 HIV患者中检出 HGV阳性 12 3例 ,阳性率为 38.8% ;91例 HCV患者中 HGV阳性 19例 ,阳性率为 2 0 .9% ,统计学分析有显著差异。进一步研究显示 ,HIV和 HGV重叠感染中 HIV病毒载量明显低于单独 HIV感染者 (1.8± 0 .6 )× 10copies/ ml vs(1.9± 1.1)× 10 2 copies/ ml];而 HCV和 HGV重叠感染者中 HCV的病毒载量与单独 HCV感染者比较没有显著差异 (1.5± 0 .6 )× 10 4copies/ ml vs(5 .4± 1.8)× 10 4copies/ ml]。序例分析表明 ,与 HIV或 HCV重叠感染的 HGV来源于同一病毒株。结论 :HIV感染者有很高的 HGV重叠感染率 ,而且 HGV感染能明显抑制 HIV在体内的病毒复制。

Influence of HGV super-infected with HIV or HCV on the virus replication

OBJECTIVE: To realize human immunodeficiency virus(HIV) and hepatitis C virus(HCV) super-infected with hepatitis G virus(HGV or GBV/C) and to probe into the mechanism of these virus infection in the body. METHODS: HIV and HCV load were tested by the quantitated RT-PCR in the HIV or HCV infected plasma samples respectively and the HGV RNA was detected in all of the samples. Then some of the HGV positive were sequenced. RESULTS: 123 of 317 HIV patients were positive for HGV, the positive rate was 38.8%. Among the 91 HCV patients, 19 were positive for HGV. The positive rate is 20.9% which was less than that of HIV patients. HIV load of the patients super-infected with HGV was less than that of those without HGV(1.8+/-0.6)x10 copies/ml compared with (1.9+/-1.1)x10(2)copies/ml]; while HGV and HCV super-infection did not influence the HCV RNA load significantly (1.5+/-0.6)x10(4) copies/ml compared with (5.4+/-1.8)x10(4)copies/ml]. The HGV sequences from HIV or HCV patients were compared and showed no difference markedly. CONCLUSION: The rate of the HIV and HGV super-infection is higher than that of HCV. HGV may inhibit HIV reproduction in the body while superinfection.