LY294002联合SN50对裸鼠胃癌模型肿瘤细胞生长和凋亡的影响

目的探讨磷脂酰肌醇3-激酶（P13K）抑制剂LY294002与NF—KBP65核转运抑制剂SN50联合应用对裸鼠荷人胃癌模型的影响。方法采用裸鼠皮下胃癌SGC7901细胞注射法建立移植瘤模型,将模型鼠分为对照组、LY294002干预组、SN50干预组、LY294002与SN50联合干预组,每组5只。干预处理10d后观察各组肿瘤大小并计算每组肿瘤抑制率。用免疫组织化学法检测Bcl-2、P53、Bax蛋白表达,并用透射电子显微镜从形态变化上观察各组肿瘤细胞凋亡情况。结果药物干预10d后,联合干预组裸鼠胃癌细胞生长抑制率为（49．2±2．5）％,明显高于LY294002干预组（29．4±1．5）％和SN50干预组（19．7±1．6）％,差异有统计学意义（P〈0．05）。此外,与LY294002干预组和SN50干预组相比,联合干预组Bcl-2表达下调和P53、Bax表达上调更为显著（P〈0．05）,胃癌细胞凋亡程度更为严重。结论LY294002与SN50联合应用可以显著抑制人胃癌SGC7901细胞裸鼠移植瘤的生长并促进胃癌细胞凋亡。

Effects of combined therapy of LY294002 and SN50 on nude mice model with gastric cancer

Objective To investigate the effect of phosphatidylinositol 3 -kinase inhibitor LY294002 combined with NF-κB P65 nuclear translocation inhibitor SN50 on the tumor cell growth and apoptosis using a nude mouse model of gastric cancer. Methods Human gastric cancer cell strain SGC7901 was transplanted subcutaneously to nude mice to establish tumor models. Model mice were randomly divided into the control group, the LY294002 treatment group, the SN50 treatment group,and the LY294002+SN50 treatment group, with 5 in each group. After being treated for 10 days, the inhibition rate of tumor growth was ascertained by measuring the size of tumor. Immunohistochemical method was used to detect the expression levels of Bcl-2, P53 and Bax proteins and transmission electron microscopy to investigate the apoptosis of tumor cells. Results On the 10th day after treatment, the inhibition rate of gastric cancer cellular growth in the LY294002+SN50 group was (49.2±2.5)%, which was significantly higher than that in the LY294002 group (29.4±1.5)% and SN50 group (19.7 ±1.6)% (P<0.05). In comparison with the other two groups, LY294002+SN50 group exhibited more severe apoptosis, with expression of Bcl-2 decreased and that of P53 and Bax increased more significantly (P<0.05). Conclusion LY294002 combined with SN50 inhibits the growth of SGC7901 transplanted tumor and aggravates the apoptosis of gastric cancer cells in nude mice model.