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In vitro cytotoxicity,cell cycle arrest,and antioxidation studies of ruthenium(II) complex [Ru(dmb)2(AHPIP)](ClO4)2 |
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| Authors: | De-Gang Xing Yan Zhang Gan-Jian Lin Yang-Yin Xie Shu-Yong Deng Hong-Liang Huang Guang-Bin Jiang Yun-Jun Liu |
| Affiliation: | 1. School of Basic Science, Guangdong Pharmaceutical University, Guangzhou, 510006, People’s Republic of China 2. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, People’s Republic of China 3. School of Life Science and Biopharmaceutical, Guangdong Pharmaceutical University, Guangzhou, 510006, People’s Republic of China |
| Abstract: | A new Ru(II) complex [Ru(dmb)2(AHPIP)](ClO4)2 (Ru1) was synthesized and characterized by elemental analysis, electrospray ionization mass spectra, and 1H NMR and 13C NMR. The cytotoxicity in vitro toward BEL-7402, HeLa, MCF-7, and MG-63 cells was studied by MTT method. The complex shows moderate cytotoxic activity and the IC50 values are 20.2 (±1.6), 16.8 (±1.3), 39.9 (±2.5), and 46.7 (±2.0) μM toward BEL-7402, HeLa, MCF-7, and MG-63 cell lines, respectively. Apoptosis was studied by acridine orange/ethidium bromide staining and flow cytometry. The cellular uptake showed that the complex can enter into the cytoplasm. The cell cycle arrest showed that Ru1 inhibits the proliferation of BEL-7402 cells in the G0/G1 phase. In addition, the antioxidant activity of the complex against hydroxyl radical (·OH) was also investigated. |
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