地黄饮子对运动性疲劳小鼠运动能力的影响

目的 探讨地黄饮子对运动性疲劳小鼠运动能力的影响.方法 将昆明小鼠30只随机分为安静对照组(安静组)、疲劳模型组(模型组)和地黄饮子组,每组10只.采用游泳训练方式建立小鼠疲劳模型.常规饲料喂养外,地黄饮子组给予地黄饮子水煎液灌胃,安静组和模型组给予蒸馏水灌胃,连续给药28 d.ELISA法检测血尿素氮、睾酮、皮质醇含...

Effect of Radix Rehmanniae Decoction on Exercise Ability in Exercise-Induced Fatigue Mice

Objective To study the effect of Radix Rehmanniae Decoction on exercise ability of mice with exercise fatigue.Methods 30 Kunming mice were randomly divided into quiet control group(quiet group),fatigue model group(model group)and Radix Rehmanniae Decoction group,and 10 mice in each group.The fatigue model of mice was established by swimming training.In addition to conventional feed feeding,the Radix Rehmanniae Decoction group was given a gavage of Radix Rehmanniae Decoction,and the quiet group and the model group were given gavage of distilled water for 28 consecutive days.The levels of blood urea nitrogen(BUN),testosterone and cortisol,the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-PX)and malondialdehyde(MDA)in liver tissue were detected by ELISA;the protein expression of adenylate activated protein kinase(AMPK)and peroxisome proliferating activated receptor coactivator-1α(PGC-1α)were detected by Western blot.Results Compared with the quiet group,the body mass of the model group was significantly increased(P<0.05);the content of serum testosterone decreased significantly(P<0.05);the contents of cortisol and BUN were significantly increased(P<0.05);the activity of GSH-PX and SOD in liver tissues was significantly decreased(P<0.05),and the content of MDA was significantly increased(P<0.05);the expression of AMPK and PGC-1αwas increased(P<0.05).Compared with the model group,the body mass of mice in the Radix Rehmanniae Decoction group increased significantly (P<0.05);the exhausted swimming time was significantly prolonged (P<0.05);the blood testosterone content was significantly increased (P<0.05);the contents of cortisol and BUN was significantly reduced (P<0.05);GSH-Px and SOD activities in liver tissue were significantly increased (P<0.05), and MDA content was significantly reduced (P<0.05);the expression of AMPK and PGC-1α was increased(P<0.05). Conclusion Radix Rehmanniae Decoction can play a role in alleviating sports fatigue to a certain extent, the mechanism may be by reducing the content of cortisol, BUN and MDA in sports fatigue model mice, increasing the activity of GSH-Px and SOD, and enhancing the regulation of AMPK / PGC-1α signaling pathway on energy metabolism, which can enhance exercise capacity of mice.