A preliminary identification of PIN1 SNP linkage in patients with coronary heart disease from Handan,China |
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Authors: | Jing-Zhang Wang Yu-Hua Zhang Jing Bai Wen-Tao Du Xiang-Yang Zhang |
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Affiliation: | 1. Affiliated Hospital, College of Medicine, Hebei University of Engineering, Handan 056002, PR China;2. College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056021, PR China |
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Abstract: | Our aim was to perform an initial assessment of the polymorphic patterns of the PIN1 gene in patients with coronary heart disease (CHD). The PIN1-encoded protein (Pin1) suppresses eNOS-NO signaling and may impair cardiovascular function. Blood collection, DNA extraction, PCR amplification and gene sequencing were performed for thirty CHD participants living in central China, focusing on nine single nucleotide polymorphisms (SNPs). Their genetic linkages were revealed and their allele frequencies were compared with SNP data from the NCBI. Three major linkage patterns were identified: [1.rs2287839-5.rs2233682], [3.rs2233679-4.rs1077220–8.rs2287838] and [6.rs889162-7.rs2010457], suggesting correlated involvement in CHD and possible simultaneous genetic origin in ancient times. The frequencies of six SNPs are consistent with the NCBI data, while the frequencies of three SNPs (2.rs2233678, 4.rs1077220 and 9.rs4804461) are not consistent with the NCBI. Especially, the 3.rs2233679–4.rs1077220 linkage is different from other populations worldwide and may be an interesting genetic characteristic of Chinese CHD patients. Predictably, 1.rs2287839, 2.rs2233678, 3.rs2233679 and 5.rs2233682 may be strongly associated with CHD risk, although this requires future verification. The PIN1 SNP linkages lay a new genetic foundation for discovering novel molecular mechanisms of CHD and for exploring PIN1-based targeted treatment of CHD with nitric oxide regulatory therapies in clinical practice. |
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Keywords: | Coronary heart disease PIN1 Single-nucleotide polymorphism Genetic linkage Endothelial nitric oxide synthase Nitric oxide Doença coronária PIN1 SNP eNOS Óxido nítrico |
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