Effects of Hangeshashinto on the nasal physiological function: An in vitro study. |
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| Authors: | Kosuke Tochigi Kazuhiro Omura Keisuke Miyashita Satoshi Aoki Nobuyoshi Otori Yasuhiro Tanaka |
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| Affiliation: | 1. Department of Otorhinolaryngology, Dokkyo Medical University Saitama Medical Center, 2-1-50 Minamikoshigaya, Koshigaya-shi, Saitama, 343-8555, Japan;2. Department of Otorhinolaryngology, The Jikei University School of Medicine, 3-19-18 Nishi-shimbashi, Minato-ku, Tokyo, 105-8471, Japan;1. Unit of Public Health and Preventive Medicine, School of Medicine, Yokohama City University, Japan;2. Department of Otorhinolaryngology, Tokai University, 143 Shimokasuya, Isehara 259-1193, Kanagawa, Japan;3. Nishiyama ENT Clinic, Japan;1. Department of Otorhinolaryngology-Head and Neck Surgery, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami, Sagamihara, Kanagawa, 252-0374, Japan;2. Department of Gastroenterology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami, Sagamihara, Kanagawa, 252-0374, Japan;3. Department of Pathology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami, Sagamihara, Kanagawa, 252-0374, Japan;4. Department of Research and Development Center for New Medical Frontiers, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami, Sagamihara, Kanagawa, 252-0374, Japan;1. Department of Otorhinolaryngology - Head and Neck Surgery, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki 569-8686, Osaka, Japan;2. Department of Pathology, Osaka Medical College, Takatsuki, Osaka, Japan;1. Department of Otolaryngology-Head and Neck Surgery, Hokuto Hospital, Inadacho Kisen 7-5, Obihiro 080-0833, Japan;2. Department of Otolaryngology-Head and Neck Surgery, Asahikawa Medical University, Midorigaoka-Higashi 2-1-1-1, Asahikawa 078-8510, Japan;3. Department of Cardiovascular Surgery, Hokuto Hospital, Inadacho Kisen 7-5, Obihiro 080-0833, Japan;4. Department of Pathology, Hokuto Hospital, Inadacho Kisen 7-5, Obihiro 080-0833, Japan;5. Keio Cancer Center, Keio University School of Medicine, 35 Shinanomachi, Shinjukuku, Tokyo 160-8582, Japan;6. Department of Surgical Pathology, Asahikawa Medical University Hospital, Midorigaoka-Higashi 2-1-1-1, Asahikawa 078-8510, Japan;1. Sen-En Rifu Otological Surgery Center, Miyagi, Japan;2. Department of Otolaryngology-Head and Neck Surgery, Tohoku University School of Medicine, 1 1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan;3. Department of Neurosurgery, Southern Tohoku General Hospital, Fukushima, Japan |
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| Abstract: | ObjectiveHangeshashinto is a Japanese Kampo medicine applied for the treatment of oral mucositis and gastroenteritis. Hangeshashinto exhibits broad-spectrum antibacterial activity and suppresses prostaglandin (PG)E2 production in the mucosa and has the ability to improve the inflammatory condition. In addition to these effects, because cAMP, a composition of Hangeshashinto, facilitates ciliary beat, Hangeshashinto could also improve the physiological function of the nasal mucosa, consist of ciliated epithelium, but details were unknown.MethodsThis study was aimed to investigate the effects of Hangeshashinto on the nasal mucosa. Healthy nasal mucosal sections were collected from the nasal septum of ten Japanese white rabbits, placed in a collagen dish for tissue culture, and rinsed with two different concentrations of Hangeshashinto solution (1.0%, n = 10 and 2.5%, n = 10) and cAMP solution (50µM, n=10 and 100 µM, n=10) or saline (control, n = 10). Ciliary beat frequency (CBF) as a physiological function of the nasal mucosa was recorded at 1, 3 and 7 days after rinsing, and histological evaluation of epithelial damage was performed at 7 days after rinsing.ResultsCBF in the 1.0% but not in the 2.5% Hangeshashinto group, increased at 3 and 7 days compared with that in the control group (p < 0.05). This trend was also observed in the CBF in the 100 µM cAMP group, significant difference was not observed between the CBF of the 1.0% Hangeshashinto group and the 100 µM cAMP group at 1, 3 and 7 days after rinsing (p > 0.05). Histological score only in the 2.5% Hangeshashinto group was lower than that in the control group (p < 0.05), while a significant decline was not observed in the other groups compared to that in the control group (p > 0.05).ConclusionOur results suggest that 1.0% Hangeshashinto solution facilitates the physiological function of the nasal mucosa by promoting ciliary functions without histological damage of cilia epithelium. When applied with the appropriate concentration, Hangeshashinto could have ability to improve the physiological functions of the nasal mucosal epithelium. |
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