推拿按法对慢性激痛点模型大鼠骨骼肌超微结构的影响

目的 观察推拿按法对慢性激痛点模型大鼠骨骼肌超微结构的影响,探讨推拿按法对慢性激痛点的治疗作用.方法 将健康雄性SD大鼠随机分为空白组、模型组、按法组、利多卡因组,每组6只.采用钝性打击结合离心运动方式建立慢性激痛点大鼠模型.按法组大鼠予按法刺激局部激痛点,隔天治疗1次,每次7.5 min,共治疗7次;利多卡因组大鼠予...

Effect of Pressing Manipulation in Massage on Ultrastructure of Skeletal Muscle in Rats with Chronic Myofascial Trigger Points

Objective To observe the effect of pressing manipulation in massage on the ultrastructure of skeletal muscle with chronic myofascial trigger points(MTrPs)in rat model,so as to explore the therapeutic effect of pressing manipulation in massage on chronic MTrPs.Methods Healthy male SD rats were randomly divided into blank group,model group,pressing manipulation group and lidocaine group,with 6 rats in each group.Rat model of chronic MTrPs was established by blunt impact combined with eccentric exercise.The rats in the pressing manipulation group were stimulated by the local MTrPs,once every other day,7.5 minutes each time,7 times in total;the rats in the lidocaine group were treated with 0.5 mL 1%lidocaine injection once every 6 days for 3 times.After 2 weeks of intervention,HE staining was used to observe the changes of skeletal muscle microstructure,and transmission electron microscope was used to observe the changes of skeletal muscle ultrastructure. Results (1) Under light microscope, abnormal fusiform muscle fibers were observed in the model group, which showed enlargement in the middle and narrowing at both ends, accompanied by a large number of inflammatory cells infiltration and nuclear internalization. Compared with the model group, the muscle fibers of the pressing manipulation group and the lidocaine group were more uniform, more orderly, and less inflammatory cells. (2) Under electron microscope, myofibrils in the model group were disordered, a large number of myofibrils were broken and damaged, the number of mitochondria was reduced, and the structure was abnormal, such as swelling, rounding, crest reduction or vacuolation. The results of the pressing manipulation group and the lidocaine group were similar. Compared with the model group, myofibril arrangement tended to be more orderly, the number of mitochondria increased, the structure returned to normal, and the myofibril was elongated, rod-shaped and ovo-shaped, or there were fusion forms. The results of myosome length measurement in each group showed that compared with the blank group, the sarcomere length of the model group was significantly shortened, and the results were significantly different (P<0.01). There were significant differences between the model group, the pressing manipulation group and the lidocaine group (P<0.05). Conclusion The pathological features of MTrPs are musculoskeletalmuscle contracture and damaged mitochondrial structure and quantity. Pressing manipulation therapy can relax the contracture of sarcomere, promote the damage repair of mitochondria, and inactivating of the chronic MTrPs.