重症肌无力患者外周血中CD4＋CD25high调节性T细胞的变化及意义

目的 研究CD4＋CD25high调节性T细胞（CD4＋CD25high regulatory T cells,CD4＋CD25highTr）在重症肌无力（myasthenia gravis,MG）发病过程中的作用.方法 采用流式细胞仪对51例MG患者外周血中CD4＋CD25highTr的表达量进行测定,分组比较病情不同的MG患者外周血中CD4＋CD25highTr的表达量,并选择35例健康体检者作为正常对照.结果 MG组患者外周血中CD4＋CD25highTr的表达量低于正常对照组的健康体检者,差异具有统计学意义（P〈0.05）;病情控制不佳的MG患者外周血中CD4＋CD25highTr的表达量低于病情稳定的MG患者及健康体检者（均P〈0.05）;胸腺切除的MG患者外周血中CD4＋CD25highTr的表达量明显高于非胸腺切除患者（P〈0.05）.结论 MG患者外周血中CD4＋CD25highTr的表达量异常,其可能参与MG的发生与发展,与MG的稳定程度密切相关.

I Changes of CD4+CD25high regulatory T cells in peripheral blood of patients with myasthenia gravis

Objective TO investigate the changes of CD4＋CD25high regulatory T cells （CD4＋CD25high Tr） in peripheral blood of patients with myasthenia gravis （MG）. Methods CD4＋CD25highTr cells in the peripheral blood were detected by flow cytometry analysis in 51 MG patients and 35 normal subjects. Results The percentage of CD4＋OD25highTr cells in peripheral blood of MG patients were higher than those of normal controls （P〈0.05） ; MG patients with uncontrolled disease （n =31） showed a lower percentage of CD4＋CD25highTr cells （2.95% ＋ 2.08%） than those of MG patients with stable disease （4.87%± 1.70 %, P〈 0.05） ; In addition, thymectomized MG patients （n =30） had significantly higher CID4＋CD25highTr cell percentage （4.03% ± 2.27%） compared with non -thymectomized MG patients （n = 21, 2.65% ± 1.74%, P〈0.05）. Conclusion These observations indicate the existence of abnormal percentage of CD4＋CD25highTr cells in peripheral blood of MG patients which may be correlated with disease progress.