系统性硬化患者内皮祖细胞连接黏附分子-A表达

目的研究系统性硬化(SSc)患者外周血内皮祖细胞连接黏附分子-A(JAM-A)表达情况。方法收集13例SSc患者及13例对照组新鲜EDTA抗凝血2ml,采用流式细胞仪方法检测,用PerCP-CY5.5、PE、Alexa Fluor 647和FITC标记CD34、CD133、CD309和JAM-A。内皮祖细胞定义为CD34、CD133、CD309(VEGFR-2,KDR)均阳性的细胞。结果内皮祖细胞表达JAM-A,SSc患者内皮祖细胞中JAM-A表达较正常对照减少(分别为0.0775±0.0385和0.1567±0.1223,P<0.05),SSc患者内皮祖细胞数量亦较对照组少(分别为0.0817±0.0403和0.1746±0.1419,P<0.05)。结论 SSc患者内皮祖细胞中JAM-A的减少是由于患者内皮祖细胞数目减少所致。SSc患者存在血管生成障碍,JAM-A表达异常,并且在SSc发病机制中起一定作用。

Expression of Junctional Adhesion Molecule-A in the Endothelial Progenitor Cells of Systemic Sclerosis Patients

To investigate the expression of junctional adhesion molecule-A in the endothelial progenitor cells of systemic sclerosis patients （SSc）. Methods We collected blood from 13 SSc patients and 13 normal control, PerCP-CY5.5 conjugated monoelonal antibody against human CD34; phycoerythrin （PE）- conjugated monoclonal antibody against human CD133; Alexa Fluor647 conjugated anti-CD309 antibody; fluorescein isothiocyanate （FITC） conjugated anti-JAM-A antibody were used to stain the cells. Flow cytometrie analysis was performed on a FACSCalibur （BS Bioseienees）. Endothelial progenitor cells （EPCs） are cells co-expressing the surface antigens CD34, CD133, and CD309. Results We found that EPCs do express JAM-A; and compared with nomal control （0. 1567 ±0. 1223）, expresion of JAM-A in EPCs are lower in SSc patients （0. 0775 ±0. 0385 ）. Conclusions Expresion of JAM-A in EPCs are lower in SSc patients because the EPCs numbers in SSc patients are lower than that in normal control. These further demonstrated defective vasculogenesis in systemic sclerosis, and it need further research on JAM-A role in SSe.