| 尼古丁对脂多糖诱导的大鼠小胶质细胞激活和白细胞介素6表达的影响 |
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| 引用本文： | 李治华,赵青赞,张华,任秀花,周明付,臧卫东.尼古丁对脂多糖诱导的大鼠小胶质细胞激活和白细胞介素6表达的影响[J].郑州大学学报(医学版),2011,46(2):188-192. |
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| 作者姓名： | 李治华 赵青赞 张华 任秀花 周明付 臧卫东 |
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| 作者单位： | 郑州大学基础医学院人体解剖学教研室,郑州,450001 |
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| 基金项目： | 国家自然科学基金资助项目30671103 |
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| 摘 要： | 目的:观察尼古丁对脂多糖(LPS)诱导的原代大鼠脑皮层小胶质细胞激活的影响及对细胞因子白细胞介素6(IL-6)表达的影响,探讨尼古丁在PD中的可能作用机制.方法:培养原代大鼠脑皮质胶质细胞,纯化小胶质细胞,用尼古丁预处理30 min,再加入LPS,采用ELISA检测小胶质细胞不同时间点分泌IL-6的水平及免疫细胞化学检...
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| 关 键 词： | 帕金森病 小胶质细胞 脂多糖 尼古丁 白细胞介素6 |
Effects of nicotine on LPS-induced microglia activation and cytokine IL-6 expression in rats |
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| LI Zhihua
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ZHAO Qingzan
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ZHANG Hua
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REN Xiuhua
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ZHOU Mingfu
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ZANG Weidong.Effects of nicotine on LPS-induced microglia activation and cytokine IL-6 expression in rats[J].Journal of Zhengzhou University: Med Sci,2011,46(2):188-192. |
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| Authors: | LI Zhihua ZHAO Qingzan ZHANG Hua REN Xiuhua ZHOU Mingfu ZANG Weidong |
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| Institution: | LI Zhihua,ZHAO Qingzan,ZHANG Hua,REN Xiuhua,ZHOU Mingfu,ZANG Weidong Department of Human Anatomy,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001 |
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| Abstract: | Aim:To observe the effects of nicotine on LPS-induced primary rat cortical microglia activation and cytokine IL-6 expression and to explore the possible mechanism of nicotine in PD.Methods:Primary rat cortical glial cells were cultured and microglial cells were purified,with or without nicotine and/or LPS.The IL-6 secretion concentration of microglia at different time was detected by ELISA and the activation of microglia(Iba1 positive cells) was detected by immunocytochemical staining.Results:LPS induced ac... |
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| Keywords: | Parkinson's disease microglia lipopolysaccharide nicotine IL-6 |
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