癫痫大鼠海马小胶质细胞的激活特点

目的:研究癫痫大鼠海马小胶质细胞在癫痫持续状态后不同时间点激活的特点。方法:采用锂-匹罗卡品癫痫大鼠及免疫组化方法比较不同时间点OX-42阳性细胞的增殖及形态变化。结果:小胶质细胞在癫痫持续状态(SE)后被激活;OX-42阳性细胞在SE 3～7 d时增加达到一个峰值,7～14 d时细胞的增加处于一个相对稳定状态,而14 d以后增加的OX-42阳性细胞逐渐下降,大约在21 d时细胞数恢复到较先前稍高的水平。结论:癫痫发作后的炎症病理损伤与小胶质细胞的增殖活化密切相关,小胶质细胞的激活是构成癫痫炎症损伤机制的重要环节。

Characteristic of microglial activation of hippocampus in experimental epileptic rats

Objective: To investigate the characteristics of microglial activation of hippocampus in experimental epileptic rata.Methods: Morphological changes and proliferation of OX-42 positive cells were compared at different time points after status of epilepticus(SE) in lithium-pilocarpine induced epileptic rats.Results: OX-42 positive cells were activated after SE,which increased to a peak at 3-7 d and in a relatively stable state at 7-14 d;then gradually decreased after 14d and returned to slightly higher level than previously at 21 d.Conclusion: Inflammatory injury,microglial activation and cell proliferation are closely related after seizures,microglial activation may be an important mechanism in the inflammatory injury of epilepsy.