荧光定量PCR检测HBV DNA及其与Pre-S1和HBeAg关系探讨

目的探讨慢性乙肝患者HBVDNA与血清标志物前S1抗原(Pre-S1)和HBeAg的相互关系。方法采用荧光定量聚合酶链反应技术(FluorescencequantitativePCR,FQ-PCR)和酶联免疫吸附试验(ELISA)对931份HBV-M不同阳性模式及35份HBV-M全阴模式血清进行HBVDNA及其标志物检测。结果在931例不同阳性模式乙肝患者中,HBeAg阳性总检出率为20.7%,Pre-S1抗原阳性总检出率为30.2%,HBVDNA阳性总检出率为45.3%。在422例HBVDNA(+)乙肝患者中,Pre-S1抗原阳性检出率为57.3%,HBeAg阳性检出率为44.1%。在281例Pre-S1(+)乙肝患者中,HBVDNA阳性符合率达86.1%,在Pre-S1(-)组HBVDNA阳性率仅为27.7%。结论HBVDNA检测是反映HBV复制最直接的分子生物学指标;Pre-S1抗原和HBeAg是反映HBV复制的血清学指标,Pre-S1抗原甚至比HBeAg更灵敏地反映HBV复制。

The analysis of the relationship between the HBV serological markers and the HBV DNA detected by FQ - PCR

Objective To investigate the relationship between the HBV serological markers and the HBV DNA in the chronically hepatitis B virus infected patients.Methods The ELISA was used to analyze these serum markers of HBV in 931 case chronic hepatitis B patients who were included in this study.The markers,like as HBsAg,anti-HBs,HBeAg,anti-HBe,anti-HBc and Pre-S1 was determined by ELISA,while the HBV DNA was determined by fluorescence quantitative PCR(FQ-PCR)assay.The relationship of Pre-S1 antigen,HBeAg and HBV DNA involved in the HBV replication was also analyzed simultaneously.Results The total positive rate of HBeAg in these patients just was 20.7%,while the total positive rate of Pre-S1 antigen in these patients was 30.2%,and the positive rate of HBV DNA reached 45.3%.We found that the total positive rate of Pre-S1 antigen was 57.3% while the total positive rate of HBeAg was just 44.1% in 422 case of HBV DNA positive patients.Meanwhile,we found that there was a significant difference of the positive rate of HBV DNA between Pre-S1( ) and Pre-S1(-)groups (P<0.01).Conclusion The HBV DNA determined by FQ-PCR is the most directly molecular biology marker,which reflects the HBV replication.Both Pre-S1 antigen and HBeAg are the more important serological markers of HBV replication.Pre-S1 is even more sensitive than HBeAg to reflect the HBV replication in chronic hepatitis B patients.