端粒酶活性与骨肉瘤细胞分化的关系

【目的】探讨人骨肉瘤细胞分化与端粒酶活性调节之间的关系。【方法】用维甲酸和地塞米松诱导人骨肉瘤细胞分化 ,用端粒重复序列扩增法 (TRAP)检测端粒酶活性及用Northern印迹法检测人端粒酶RNA(RNA ,hTR)表达水平的改变。【结果】经维甲酸和地塞米松处理后 ,骨肉瘤细胞生长受到抑制 ,碱性磷酸酶活性升高 ,端粒酶活性明显下降 ,且与剂量 作用时间成负相关 ,但hTR表达水平并无改变。【结论】维甲酸和地塞米松有抑制骨肉瘤细胞增殖和诱导分化的作用 ,端粒酶活性下调可作为细胞分化指标 ,但与hTR表达水平不平行。

Study on the Correlation of Telomerase Activity and Differentiation of Cultured Osteosarcoma Cells

Objective To investigate the relationship between induced differentiation and regulation of telomerase activity in cultured osteosarcoma cells. Methods Osteosarcoma cells were treated by different doses of retinoic acid (RA) and dexamethasone (DEX). Telomerase activity and human telomerase RNA (hTR) of treated cells were detected by telomere repeat amplification protocol (TRAP) and by Northern blotting analysis. Results After treatment with RA or DEX, the growth of HOS cells was inhibited. Alkaline phosphatase activity (differentiation marker of osteogenetic cells) of the cells increased significantly. Telomerase activity decreased with increasing treatment doses and times, but the expression level of hTR did not change obviously. Conclusions Both RA and DEX could inhibit cell growth and induce differentiation of HOS cells. Decreasing of telomerase activity could be considered as a marker of differentiation of HOS cells, but it does not correlate with the expression level of hTR.