转染Ang-1间充质干细胞加强兔骨缺损修复的实验研究

目的 研究转染血管生成素-l(angiopoiten—1．Ang—1)基因的骨髓间充质干细胞(bone-mesenchvmal stem cells，BMSCs)对免骨缺损的修复作用。方法以脂质体介导pCDNA3．Ang—1质粒转染体外分离培养的兔BMSCs，与TCP陶瓷复合，修复兔桡骨15mm长节段性骨缺损?以未转染细胞作对照，进行电镜、组织学及核素扫描等方法检测骨修复情况。结果细胞与TCP材料复合后生长良好。组织学检查见转染细胞组毛细血管生长及新骨形成活跃，核素扫描见实验组局部血供丰富、代谢旺盛。结论Ang—l转染细胞组血供增加和骨修复加速，与细胞因子局部定向释放，加强血管形成及骨代谢有关。Ang—1修饰的BMSCs预构人工骨有助于加速骨缺损的修复?

An experimental study on bone mesenchymal stem cells transfected with Ang-1 gene in accelerating bone repair in rabbits

Objective To study the effect of bone mesenchymal stem cells (BMSCs) transfected with Ang 1 gene on repairing rabbit bone defects. Methods The rabbit BMSCs cultured in vitro were transfected by eukaryotic expression vector pCDNA3 hAng 1 with liposome DOTAP, seeded into biodegradable porous tricalcium phosphate (TCP) ceramics and then grafted to fill the segmental radial defects of 15mm in length. The untransfected BMSCs were taken as control. The bone repair was observed by regular electro microscopic scanning, histological and SPECT studies. Results BMSCs grew well in the TCP ceramics. In the experiment group, histological examination demonstrated more capillary regeneration and active new bone formation, and SPECT showed more abundant blood supply and more active metabolism. Conclusions The increase of blood supply and acceleration of bone repair in the experiment group can be related to the oriented release of cell factors, the increase of osteogenesis and bone metabolism. The use of artificial bone pre constructed by BMSCs transfected with Ang 1 can accelerate bone repair.